KEY FEATURES
| Full Name | Apolipoprotein C2 |
|---|---|
| Synonym | APC2; Apo-C2; ProapoC-II; Apolipoprotein C-II; Proapolipoprotein C-II |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 7.8-500ng/mL |
| Sensitivity | 2.9ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human APOC2 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human APOC2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human APOC2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human APOC2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human APOC2. You can calculate the concentration of Human APOC2 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human APOC2 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 27.03 | 73.9 | 171.9 | 26.44 | 74.13 | 156.76 |
| Standard deviation | 1.78 | 3.55 | 6.07 | 1.65 | 4.39 | 5.82 |
| CV(%) | 6.58 | 4.81 | 3.53 | 6.25 | 5.92 | 3.71 |
RECOVERY
The recovery of Human APOC2 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 84-98 | 91 |
| EDTA plasma (n=8) | 95-110 | 101 |
| Cell culture media (n=8) | 91-106 | 98 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human APOC2 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with ApoC2 ELISA Kits
| APOE ELISA Kit | publications with ApoC2 and APOE |
| LPL ELISA Kit | publications with ApoC2 and LPL |
| APOB ELISA Kit | publications with ApoC2 and APOB |
| APOA5 ELISA Kit | publications with ApoC2 and APOA5 |
| APOC3 ELISA Kit | publications with ApoC2 and APOC3 |
| APOA1 ELISA Kit | publications with ApoC2 and APOA1 |
| APOA2 ELISA Kit | publications with ApoC2 and APOA2 |
| APOA4 ELISA Kit | publications with ApoC2 and APOA4 |
Pathways associated with ApoC2 ELISA Kit
| Chylomicron-mediated Lipid Transport Pathway | Chylomicron-mediated Lipid Transport Pathway |
| Disease Pathway | Disease Pathway |
| Diseases Associated With Visual Transduction Pathway | Diseases Associated With Visual Transduction Pathway |
| HDL-mediated Lipid Transport Pathway | HDL-mediated Lipid Transport Pathway |
| Lipid Digestion, Mobilization, And Transport Pathway | Lipid Digestion, Mobilization, And Transport Pathway |
| Lipoprotein Metabolism Pathway | Lipoprotein Metabolism Pathway |
| Metabolism Pathway | Metabolism Pathway |
| Metabolism Of Lipids And Lipoproteins Pathway | Metabolism Of Lipids And Lipoproteins Pathway |
| Retinoid Metabolism And Transport Pathway | Retinoid Metabolism And Transport Pathway |
| Signal Transduction Pathway | Signal Transduction Pathway |
Diseases associated with ApoC2 ELISA Kit
| Dyslipidemias | publications with ApoC2 and Dyslipidemias |
| Cardiovascular Diseases | publications with ApoC2 and Cardiovascular Diseases |
| Atherosclerosis | publications with ApoC2 and Atherosclerosis |
| Hyperlipoproteinemia Type I | publications with ApoC2 and Hyperlipoproteinemia Type I |
| Liver Diseases | publications with ApoC2 and Liver Diseases |
| Kidney Diseases | publications with ApoC2 and Kidney Diseases |
| Fatty Liver | publications with ApoC2 and Fatty Liver |
| Brain Diseases | publications with ApoC2 and Brain Diseases |
| Obesity | publications with ApoC2 and Obesity |
| Inflammation | publications with ApoC2 and Inflammation |
Organs/Tissues associated with ApoC2 ELISA Kit
| Liver | publications with ApoC2 and Liver |
| Heart | publications with ApoC2 and Heart |
| Kidney | publications with ApoC2 and Kidney |
| Intestine | publications with ApoC2 and Intestine |
| Lung | publications with ApoC2 and Lung |
| Brain | publications with ApoC2 and Brain |
| Skin | publications with ApoC2 and Skin |
| Ovary | publications with ApoC2 and Ovary |
| Embryonic Tissue | publications with ApoC2 and Embryonic Tissue |
| Eye | publications with ApoC2 and Eye |
