KEY FEATURES
| Full Name | Apolipoprotein B48 |
|---|---|
| Synonym | Apo-B48 |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 2.4-150ng/mL |
| Sensitivity | 1.1ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human ApoB48 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ApoB48. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoB48 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoB48, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ApoB48. You can calculate the concentration of Human ApoB48 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human ApoB48 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 7.66 | 13.58 | 58.06 | 7.8 | 12.83 | 60.25 |
| Standard deviation | 0.44 | 0.64 | 2.44 | 0.42 | 0.59 | 2.27 |
| CV(%) | 5.69 | 4.72 | 4.21 | 5.42 | 4.56 | 3.77 |
RECOVERY
The recovery of Human ApoB48 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 97-112 | 102 |
| EDTA plasma (n=8) | 89-106 | 96 |
| Cell culture media (n=8) | 92-106 | 99 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human ApoB48 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with APOM ELISA Kits
| JUN ELISA Kit | publications with APOM and JUN |
| HNF1A ELISA Kit | publications with APOM and HNF1A |
| FOXA2 ELISA Kit | publications with APOM and FOXA2 |
| NR5A2 ELISA Kit | publications with APOM and NR5A2 |
| BAT4 ELISA Kit | publications with APOM and BAT4 |
Diseases associated with APOM ELISA Kit
| Liver Diseases | publications with APOM and Liver Diseases |
| Inflammation | publications with APOM and Inflammation |
| Liver Neoplasms | publications with APOM and Liver Neoplasms |
| Heart Diseases | publications with APOM and Heart Diseases |
| Carcinoma, Hepatocellular | publications with APOM and Carcinoma, Hepatocellular |
| Kidney Diseases | publications with APOM and Kidney Diseases |
| Dyslipidemias | publications with APOM and Dyslipidemias |
| Nervous System Diseases | publications with APOM and Nervous System Diseases |
| Hyperglycemia | publications with APOM and Hyperglycemia |
| Brain Diseases | publications with APOM and Brain Diseases |
Organs/Tissues associated with APOM ELISA Kit
| Blood | publications with APOM and Blood |
| Liver | publications with APOM and Liver |
| Kidney | publications with APOM and Kidney |
| Heart | publications with APOM and Heart |
| Lung | publications with APOM and Lung |
| Brain | publications with APOM and Brain |
| Embryonic Tissue | publications with APOM and Embryonic Tissue |
| Lymph Node | publications with APOM and Lymph Node |
| Spleen | publications with APOM and Spleen |
| Testis | publications with APOM and Testis |
