KEY FEATURES
| Full Name | Bone Morphogenetic Protein 9 |
|---|---|
| Synonym | GDF2; Growth Differentiation Factor 2 |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 62.5-4000pg/mL |
| Sensitivity | 25.2pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human BMP9 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BMP9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BMP9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BMP9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BMP9. You can calculate the concentration of Human BMP9 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human BMP9 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 195.39 | 536.27 | 1971.61 | 177.17 | 580.09 | 2164.48 |
| Standard deviation | 11.06 | 31.21 | 90.5 | 9.44 | 23.49 | 95.67 |
| CV(%) | 5.66 | 5.82 | 4.59 | 5.33 | 4.05 | 4.42 |
RECOVERY
The recovery of Human BMP9 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 90-101 | 96 |
| EDTA plasma (n=8) | 88-100 | 95 |
| Cell culture media (n=8) | 89-103 | 95 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human BMP9 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with GDF2 ELISA Kits
| ACVRL1 ELISA Kit | publications with GDF2 and ACVRL1 |
| BMPR2 ELISA Kit | publications with GDF2 and BMPR2 |
| ENG ELISA Kit | publications with GDF2 and ENG |
| HAMP ELISA Kit | publications with GDF2 and HAMP |
| SMAD5 ELISA Kit | publications with GDF2 and SMAD5 |
| CHAT ELISA Kit | publications with GDF2 and CHAT |
| HEY1 ELISA Kit | publications with GDF2 and HEY1 |
| SMAD9 ELISA Kit | publications with GDF2 and SMAD9 |
Pathways associated with GDF2 ELISA Kit
| ALK1 Signaling Events Pathway | ALK1 Signaling Events Pathway |
Diseases associated with GDF2 ELISA Kit
| Cardiovascular Abnormalities | publications with GDF2 and Cardiovascular Abnormalities |
| Disease Models, Animal | publications with GDF2 and Disease Models, Animal |
| Lung Diseases | publications with GDF2 and Lung Diseases |
| Liver Neoplasms | publications with GDF2 and Liver Neoplasms |
| Carcinoma, Hepatocellular | publications with GDF2 and Carcinoma, Hepatocellular |
| Intestinal Diseases | publications with GDF2 and Intestinal Diseases |
| Heart Diseases | publications with GDF2 and Heart Diseases |
| Fibrosis | publications with GDF2 and Fibrosis |
| Heart Failure | publications with GDF2 and Heart Failure |
| Inflammation | publications with GDF2 and Inflammation |
Organs/Tissues associated with GDF2 ELISA Kit
| Liver | publications with GDF2 and Liver |
