KEY FEATURES
| Full Name | Complement Component 1, R Subcomponent |
|---|---|
| Synonym | C1-r; complement Component 1; Complement component 1 subcomponent r |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.31-20ng/mL |
| Sensitivity | 0.15ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human C1r in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C1r. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C1r and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C1r, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C1r. You can calculate the concentration of Human C1r in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human C1r ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.96 | 3.08 | 8.54 | 1.03 | 3.37 | 7.96 |
| Standard deviation | 0.07 | 0.17 | 0.37 | 0.05 | 0.16 | 0.33 |
| CV(%) | 6.91 | 5.56 | 4.39 | 5.26 | 4.88 | 4.19 |
RECOVERY
The recovery of Human C1r spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 95-109 | 102 |
| EDTA plasma (n=8) | 88-100 | 94 |
| Cell culture media (n=8) | 92-104 | 98 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human C1r in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with C1r ELISA Kits
| C1S ELISA Kit | publications with C1r and C1S |
| CRP ELISA Kit | publications with C1r and CRP |
| SERPING1 ELISA Kit | publications with C1r and SERPING1 |
| C1QB ELISA Kit | publications with C1r and C1QB |
| C1QC ELISA Kit | publications with C1r and C1QC |
| C1QA ELISA Kit | publications with C1r and C1QA |
Pathways associated with C1r ELISA Kit
| Classical Antibody-mediated Complement Activation Pathway | Classical Antibody-mediated Complement Activation Pathway |
| Complement Activation, Classical Pathway | Complement Activation, Classical Pathway |
| Complement And Coagulation Cascades Pathway | Complement And Coagulation Cascades Pathway |
| Complement Cascade Pathway | Complement Cascade Pathway |
| Creation Of C4 And C2 Activators Pathway | Creation Of C4 And C2 Activators Pathway |
| Immune System Pathway | Immune System Pathway |
| Initial Triggering Of Complement Pathway | Initial Triggering Of Complement Pathway |
| Innate Immune System Pathway | Innate Immune System Pathway |
Diseases associated with C1r ELISA Kit
| COMPLEMENT COMPONENT C1r/C1s DEFICIENCY | publications with C1r and COMPLEMENT COMPONENT C1r/C1s DEFICIENCY |
| Cardiovascular Diseases | publications with C1r and Cardiovascular Diseases |
| Skin Diseases | publications with C1r and Skin Diseases |
| Inflammation | publications with C1r and Inflammation |
| Kidney Diseases | publications with C1r and Kidney Diseases |
| Necrosis | publications with C1r and Necrosis |
| Liver Diseases | publications with C1r and Liver Diseases |
| Edema | publications with C1r and Edema |
| Disease Models, Animal | publications with C1r and Disease Models, Animal |
| Heart Diseases | publications with C1r and Heart Diseases |
Organs/Tissues associated with C1r ELISA Kit
| Blood | publications with C1r and Blood |
| Bone | publications with C1r and Bone |
| Liver | publications with C1r and Liver |
| Skin | publications with C1r and Skin |
| Vascular | publications with C1r and Vascular |
| Brain | publications with C1r and Brain |
| Kidney | publications with C1r and Kidney |
| Embryonic Tissue | publications with C1r and Embryonic Tissue |
| Lung | publications with C1r and Lung |
| Muscle | publications with C1r and Muscle |
