KEY FEATURES
| Full Name | Complement Component 4b |
|---|---|
| Synonym | C4-B;CPAMD3;C4(Chido Blood Group);Basic C4; |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 1.25-80ng/mL |
| Sensitivity | 0.55ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human C4b in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C4b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C4b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C4b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C4b. You can calculate the concentration of Human C4b in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human C4b ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 4.34 | 12.23 | 38.59 | 4.14 | 13.3 | 35.95 |
| Standard deviation | 0.23 | 0.52 | 2.06 | 0.24 | 0.69 | 1.7 |
| CV(%) | 5.39 | 4.22 | 5.33 | 5.9 | 5.22 | 4.74 |
RECOVERY
The recovery of Human C4b spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-105 | 99 |
| EDTA plasma (n=8) | 83-98 | 90 |
| Cell culture media (n=8) | 85-99 | 92 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human C4b in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with C4 ELISA Kits
| C4B ELISA Kit | publications with C4 and C4B |
| C4BPB ELISA Kit | publications with C4 and C4BPB |
| PROS1 ELISA Kit | publications with C4 and PROS1 |
| APCS ELISA Kit | publications with C4 and APCS |
| CFI ELISA Kit | publications with C4 and CFI |
| CD40 ELISA Kit | publications with C4 and CD40 |
Pathways associated with C4 ELISA Kit
| CD40/CD40L Signaling Pathway | CD40/CD40L Signaling Pathway |
| Complement And Coagulation Cascades Pathway | Complement And Coagulation Cascades Pathway |
| Complement Cascade Pathway | Complement Cascade Pathway |
| Immune System Pathway | Immune System Pathway |
| Innate Immune System Pathway | Innate Immune System Pathway |
| Pertussis Pathway | Pertussis Pathway |
| Regulation Of Complement Cascade Pathway | Regulation Of Complement Cascade Pathway |
Organs/Tissues associated with C4 ELISA Kit
| Blood | publications with C4 and Blood |
| Liver | publications with C4 and Liver |
| Uterus | publications with C4 and Uterus |
| Lung | publications with C4 and Lung |
| Muscle | publications with C4 and Muscle |
| Brain | publications with C4 and Brain |
| Intestine | publications with C4 and Intestine |
| Connective Tissue | publications with C4 and Connective Tissue |
| Mammary Gland | publications with C4 and Mammary Gland |
