KEY FEATURES
| Full Name | Cluster of Differentiation 90 |
|---|---|
| Synonym | Thy1; CDw90; THY mocyte differentiation antigen 1 |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 12.5-800pg/mL |
| Sensitivity | 4.7pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human CD90 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD90. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD90 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD90, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CD90. You can calculate the concentration of Human CD90 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human CD90 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 37.72 | 114.3 | 301.02 | 41.02 | 120.55 | 272.2 |
| Standard deviation | 2.63 | 6.42 | 9.63 | 2.15 | 7.1 | 12.17 |
| CV(%) | 6.96 | 5.62 | 3.2 | 5.23 | 5.89 | 4.47 |
RECOVERY
The recovery of Human CD90 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 92-104 | 97 |
| EDTA plasma (n=8) | 91-105 | 98 |
| Cell culture media (n=8) | 85-98 | 90 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human CD90 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with Thy1 ELISA Kits
| ITGAM ELISA Kit | publications with Thy1 and ITGAM |
| ITGB2 ELISA Kit | publications with Thy1 and ITGB2 |
| MAPK1 ELISA Kit | publications with Thy1 and MAPK1 |
| STAT6 ELISA Kit | publications with Thy1 and STAT6 |
Pathways associated with Thy1 ELISA Kit
| Cardiac Progenitor Differentiation Pathway | Cardiac Progenitor Differentiation Pathway |
| IL4-mediated Signaling Events Pathway | IL4-mediated Signaling Events Pathway |
| Leukocyte Transendothelial Migration Pathway | Leukocyte Transendothelial Migration Pathway |
| Amb2 Integrin Signaling Pathway | Amb2 Integrin Signaling Pathway |
Diseases associated with Thy1 ELISA Kit
| Disease Models, Animal | publications with Thy1 and Disease Models, Animal |
| Kidney Diseases | publications with Thy1 and Kidney Diseases |
| Nervous System Diseases | publications with Thy1 and Nervous System Diseases |
| Brain Diseases | publications with Thy1 and Brain Diseases |
| Inflammation | publications with Thy1 and Inflammation |
| Neoplasms, Experimental | publications with Thy1 and Neoplasms, Experimental |
| Fibrosis | publications with Thy1 and Fibrosis |
| Necrosis | publications with Thy1 and Necrosis |
| Nerve Degeneration | publications with Thy1 and Nerve Degeneration |
| Liver Diseases | publications with Thy1 and Liver Diseases |
Organs/Tissues associated with Thy1 ELISA Kit
| Blood | publications with Thy1 and Blood |
| Spleen | publications with Thy1 and Spleen |
| Bone | publications with Thy1 and Bone |
| Kidney | publications with Thy1 and Kidney |
| Bone Marrow | publications with Thy1 and Bone Marrow |
| Brain | publications with Thy1 and Brain |
| Nerve | publications with Thy1 and Nerve |
| Thymus | publications with Thy1 and Thymus |
| Liver | publications with Thy1 and Liver |
| Eye | publications with Thy1 and Eye |
