KEY FEATURES
| Full Name | Complement Factor I |
|---|---|
| Synonym | CF-I; C3b-INA; FI; IF; KAF; Factor I; Konglutinogen-Activating Factor; C3b-Inactivator; C3B/C4B inactivator |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 3.12-200ng/mL |
| Sensitivity | 1.33ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human CFI in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CFI. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CFI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CFI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CFI. You can calculate the concentration of Human CFI in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human CFI ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 10.63 | 29.6 | 89.81 | 10.95 | 31.36 | 85.71 |
| Standard deviation | 0.63 | 1.23 | 3.96 | 0.65 | 1.26 | 4.18 |
| CV(%) | 5.97 | 4.14 | 4.41 | 5.93 | 4.02 | 4.88 |
RECOVERY
The recovery of Human CFI spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-105 | 96 |
| EDTA plasma (n=8) | 85-98 | 92 |
| Cell culture media (n=8) | 85-99 | 91 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human CFI in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with CFI ELISA Kits
| CFH ELISA Kit | publications with CFI and CFH |
| CD46 ELISA Kit | publications with CFI and CD46 |
| SERPINC1 ELISA Kit | publications with CFI and SERPINC1 |
| CR1 ELISA Kit | publications with CFI and CR1 |
| C4BPA ELISA Kit | publications with CFI and C4BPA |
Pathways associated with CFI ELISA Kit
| Complement And Coagulation Cascades Pathway | Complement And Coagulation Cascades Pathway |
| Complement Cascade Pathway | Complement Cascade Pathway |
| Immune System Pathway | Immune System Pathway |
| Innate Immune System Pathway | Innate Immune System Pathway |
| Regulation Of Complement Cascade Pathway | Regulation Of Complement Cascade Pathway |
| Staphylococcus Aureus Infection Pathway | Staphylococcus Aureus Infection Pathway |
Diseases associated with CFI ELISA Kit
| Cardiovascular Diseases | publications with CFI and Cardiovascular Diseases |
| Heart Diseases | publications with CFI and Heart Diseases |
| Kidney Diseases | publications with CFI and Kidney Diseases |
| Anemia | publications with CFI and Anemia |
| Anemia, Hemolytic | publications with CFI and Anemia, Hemolytic |
| Brain Diseases | publications with CFI and Brain Diseases |
| Inflammation | publications with CFI and Inflammation |
| Atypical hemolytic uremic syndrome | publications with CFI and Atypical hemolytic uremic syndrome |
| Macular Degeneration | publications with CFI and Macular Degeneration |
| Liver Diseases | publications with CFI and Liver Diseases |
Organs/Tissues associated with CFI ELISA Kit
| Blood | publications with CFI and Blood |
| Heart | publications with CFI and Heart |
| Vascular | publications with CFI and Vascular |
| Brain | publications with CFI and Brain |
| Kidney | publications with CFI and Kidney |
| Lung | publications with CFI and Lung |
| Liver | publications with CFI and Liver |
| Eye | publications with CFI and Eye |
| Skin | publications with CFI and Skin |
| Muscle | publications with CFI and Muscle |
