KEY FEATURES
| Full Name | Connexin 43 |
|---|---|
| Synonym | ODDD; GJAL; GJA1; GJ-A1; Gap junction 43 kDa heart protein; Gap Junction Protein Alpha 1; Oculodentodigital Dysplasia; Syndactyly Type III |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.15-10ng/mL |
| Sensitivity | 0.07ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human CX43 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CX43. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CX43 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CX43, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CX43. You can calculate the concentration of Human CX43 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human CX43 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.46 | 1.47 | 3.85 | 0.48 | 1.42 | 4.21 |
| Standard deviation | 0.02 | 0.07 | 0.19 | 0.03 | 0.06 | 0.14 |
| CV(%) | 5.35 | 4.59 | 4.84 | 6.49 | 4.04 | 3.23 |
RECOVERY
The recovery of Human CX43 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-107 | 101 |
| EDTA plasma (n=8) | 92-109 | 99 |
| Cell culture media (n=8) | 84-99 | 91 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human CX43 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with GJA1 ELISA Kits
| NOV ELISA Kit | publications with GJA1 and NOV |
| TJP1 ELISA Kit | publications with GJA1 and TJP1 |
| SRC ELISA Kit | publications with GJA1 and SRC |
| GJA3 ELISA Kit | publications with GJA1 and GJA3 |
| CAV1 ELISA Kit | publications with GJA1 and CAV1 |
| MAPK1 ELISA Kit | publications with GJA1 and MAPK1 |
| GJA5 ELISA Kit | publications with GJA1 and GJA5 |
| CSK ELISA Kit | publications with GJA1 and CSK |
| PRKCG ELISA Kit | publications with GJA1 and PRKCG |
| PRKCA ELISA Kit | publications with GJA1 and PRKCA |
Pathways associated with GJA1 ELISA Kit
| Arrhythmogenic Right Ventricular Cardiomyopathy Pathway | Arrhythmogenic Right Ventricular Cardiomyopathy Pathway |
| Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway | Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway |
| Calcium Regulation In The Cardiac Cell Pathway | Calcium Regulation In The Cardiac Cell Pathway |
| EGFR1 Signaling Pathway | EGFR1 Signaling Pathway |
| Formation Of Annular Gap Junctions Pathway | Formation Of Annular Gap Junctions Pathway |
| Gap Junction Pathway | Gap Junction Pathway |
| Gap Junction Assembly Pathway | Gap Junction Assembly Pathway |
| Gap Junction Degradation Pathway | Gap Junction Degradation Pathway |
Diseases associated with GJA1 ELISA Kit
| Cardiovascular Diseases | publications with GJA1 and Cardiovascular Diseases |
| Heart Diseases | publications with GJA1 and Heart Diseases |
| Oculodentodigital Dysplasia | publications with GJA1 and Oculodentodigital Dysplasia |
| Neoplasms | publications with GJA1 and Neoplasms |
| Arrhythmias, Cardiac | publications with GJA1 and Arrhythmias, Cardiac |
| Disease Models, Animal | publications with GJA1 and Disease Models, Animal |
| Cardiomyopathies | publications with GJA1 and Cardiomyopathies |
| Fibrosis | publications with GJA1 and Fibrosis |
| Infertility, Male | publications with GJA1 and Infertility, Male |
| Breast Neoplasms | publications with GJA1 and Breast Neoplasms |
Organs/Tissues associated with GJA1 ELISA Kit
| Heart | publications with GJA1 and Heart |
| Muscle | publications with GJA1 and Muscle |
| Eye | publications with GJA1 and Eye |
| Bone | publications with GJA1 and Bone |
| Brain | publications with GJA1 and Brain |
| Vascular | publications with GJA1 and Vascular |
| Ovary | publications with GJA1 and Ovary |
| Skin | publications with GJA1 and Skin |
| Embryonic Tissue | publications with GJA1 and Embryonic Tissue |
| Testis | publications with GJA1 and Testis |
