KEY FEATURES
| Full Name | Epithelial Neutrophil Activating Peptide 78 |
|---|---|
| Synonym | CXCL5, SCYB5 |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 31.25-2000pg/mL |
| Sensitivity | 18.75pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human ENA-78/CXCL5 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ENA-78/CXCL5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ENA-78/CXCL5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ENA-78/CXCL5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ENA-78/CXCL5. You can calculate the concentration of Human ENA-78/CXCL5 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human ENA-78/CXCL5 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 108.05 | 188.74 | 968.97 | 116.22 | 188.28 | 925.61 |
| Standard deviation | 5.93 | 8.97 | 30.13 | 8.04 | 8.79 | 32.21 |
| CV(%) | 5.49 | 4.75 | 3.11 | 6.92 | 4.67 | 3.48 |
RECOVERY
The recovery of Human ENA-78/CXCL5 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-104 | 96 |
| EDTA plasma (n=8) | 94-110 | 100 |
| Cell culture media (n=8) | 93-111 | 101 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human ENA-78/CXCL5 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with CXCL5 ELISA Kits
| CXCR2 ELISA Kit | publications with CXCL5 and CXCR2 |
| CCL5 ELISA Kit | publications with CXCL5 and CCL5 |
| CCL20 ELISA Kit | publications with CXCL5 and CCL20 |
| CXCR1 ELISA Kit | publications with CXCL5 and CXCR1 |
| CXCL11 ELISA Kit | publications with CXCL5 and CXCL11 |
| CXCL16 ELISA Kit | publications with CXCL5 and CXCL16 |
| CCR3 ELISA Kit | publications with CXCL5 and CCR3 |
| CCR1 ELISA Kit | publications with CXCL5 and CCR1 |
| CCR5 ELISA Kit | publications with CXCL5 and CCR5 |
| CCL19 ELISA Kit | publications with CXCL5 and CCL19 |
Pathways associated with CXCL5 ELISA Kit
| Chemokine Receptors Bind Chemokines Pathway | Chemokine Receptors Bind Chemokines Pathway |
| Chemokine Signaling Pathway | Chemokine Signaling Pathway |
| Class A/1 (Rhodopsin-like Receptors) Pathway | Class A/1 (Rhodopsin-like Receptors) Pathway |
| Cytokine-cytokine Receptor Interaction Pathway | Cytokine-cytokine Receptor Interaction Pathway |
| G Alpha (i) Signalling Events Pathway | G Alpha (i) Signalling Events Pathway |
| GPCR Downstream Signaling Pathway | GPCR Downstream Signaling Pathway |
| GPCR Ligand Binding Pathway | GPCR Ligand Binding Pathway |
| Peptide Ligand-binding Receptors Pathway | Peptide Ligand-binding Receptors Pathway |
Diseases associated with CXCL5 ELISA Kit
| Inflammation | publications with CXCL5 and Inflammation |
| Necrosis | publications with CXCL5 and Necrosis |
| Respiratory Tract Diseases | publications with CXCL5 and Respiratory Tract Diseases |
| Lung Diseases | publications with CXCL5 and Lung Diseases |
| Disease Models, Animal | publications with CXCL5 and Disease Models, Animal |
| Carcinoma | publications with CXCL5 and Carcinoma |
| Cardiovascular Diseases | publications with CXCL5 and Cardiovascular Diseases |
| Fibrosis | publications with CXCL5 and Fibrosis |
| Lung Neoplasms | publications with CXCL5 and Lung Neoplasms |
| Liver Diseases | publications with CXCL5 and Liver Diseases |
Organs/Tissues associated with CXCL5 ELISA Kit
| Blood | publications with CXCL5 and Blood |
| Lung | publications with CXCL5 and Lung |
| Vascular | publications with CXCL5 and Vascular |
| Intestine | publications with CXCL5 and Intestine |
| Skin | publications with CXCL5 and Skin |
| Connective Tissue | publications with CXCL5 and Connective Tissue |
| Embryonic Tissue | publications with CXCL5 and Embryonic Tissue |
| Stomach | publications with CXCL5 and Stomach |
| Uterus | publications with CXCL5 and Uterus |
| Brain | publications with CXCL5 and Brain |
