KEY FEATURES
| Full Name | Glucagon Like Peptide 1 |
|---|---|
| Synonym | GLP1 |
| Assay Type | Competitive |
| Reactivity | Human |
| Range | 12.35-1000pg/mL |
| Sensitivity | 4.92pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 50μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human GLP-1 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human GLP-1. During the reaction, Human GLP-1 in the sample or standard competes with a fixed amount of Human GLP-1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human GLP-1. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human GLP-1 in tested samples can be calculated by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human GLP-1 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 46.8 | 154.69 | 447.54 | 46.93 | 161.4 | 465.77 |
| Standard deviation | 3.21 | 7.84 | 21.88 | 2.48 | 7.75 | 14.07 |
| CV(%) | 6.86 | 5.07 | 4.89 | 5.29 | 4.8 | 3.02 |
RECOVERY
The recovery of Human GLP-1 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-100 | 95 |
| EDTA plasma (n=8) | 90-102 | 96 |
| Cell culture media (n=8) | 83-98 | 90 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human GLP-1 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with GC ELISA Kits
| GLP1R ELISA Kit | publications with GC and GLP1R |
| GCGR ELISA Kit | publications with GC and GCGR |
| GLP2R ELISA Kit | publications with GC and GLP2R |
| FOXA2 ELISA Kit | publications with GC and FOXA2 |
| GIPR ELISA Kit | publications with GC and GIPR |
| SCT ELISA Kit | publications with GC and SCT |
Pathways associated with GC ELISA Kit
| Class B/2 (Secretin Family Receptors) Pathway | Class B/2 (Secretin Family Receptors) Pathway |
| FOXA1 Transcription Factor Network Pathway | FOXA1 Transcription Factor Network Pathway |
| G Alpha (q) Signalling Events Pathway | G Alpha (q) Signalling Events Pathway |
| G Alpha (s) Signalling Events Pathway | G Alpha (s) Signalling Events Pathway |
| GPCR Downstream Signaling Pathway | GPCR Downstream Signaling Pathway |
| GPCR Ligand Binding Pathway | GPCR Ligand Binding Pathway |
| Gastrin-CREB Signalling Pathway Via PKC And MAPK | Gastrin-CREB Signalling Pathway Via PKC And MAPK |
| Glucagon Signaling In Metabolic Regulation Pathway | Glucagon Signaling In Metabolic Regulation Pathway |
| Glucagon-type Ligand Receptors Pathway | Glucagon-type Ligand Receptors Pathway |
| Incretin Synthesis, Secretion, And Inactivation Pathway | Incretin Synthesis, Secretion, And Inactivation Pathway |
Diseases associated with GC ELISA Kit
| Muscular Diseases | publications with GC and Muscular Diseases |
| Diabetes Mellitus | publications with GC and Diabetes Mellitus |
| Vascular Diseases | publications with GC and Vascular Diseases |
| Obesity | publications with GC and Obesity |
| Kidney Diseases | publications with GC and Kidney Diseases |
| Insulin Resistance | publications with GC and Insulin Resistance |
| Hyperglycemia | publications with GC and Hyperglycemia |
| Heart Diseases | publications with GC and Heart Diseases |
| Drug Toxicity | publications with GC and Drug Toxicity |
| Liver Diseases | publications with GC and Liver Diseases |
Organs/Tissues associated with GC ELISA Kit
| Pancreas | publications with GC and Pancreas |
| Intestine | publications with GC and Intestine |
