KEY FEATURES
| Full Name | Inducible T-Cell Co Stimulator |
|---|---|
| Synonym | CD278; AILIM; Inducible T-Cell Costimulator; Activation-Inducible Lymphocyte Immunomediatory Molecule |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 15.6-1000pg/mL |
| Sensitivity | 6.2pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human ICOS in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ICOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ICOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ICOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ICOS. You can calculate the concentration of Human ICOS in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human ICOS ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 53.43 | 151.11 | 458.76 | 58.7 | 152.62 | 440.64 |
| Standard deviation | 3.34 | 8.22 | 22.53 | 2.99 | 8.88 | 18.99 |
| CV(%) | 6.26 | 5.44 | 4.91 | 5.1 | 5.82 | 4.31 |
RECOVERY
The recovery of Human ICOS spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-103 | 96 |
| EDTA plasma (n=8) | 84-97 | 91 |
| Cell culture media (n=8) | 91-106 | 98 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human ICOS in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with ICOS ELISA Kits
| CTLA4 ELISA Kit | publications with ICOS and CTLA4 |
| ICOSLG ELISA Kit | publications with ICOS and ICOSLG |
| RC3H1 ELISA Kit | publications with ICOS and RC3H1 |
| PIK3CD ELISA Kit | publications with ICOS and PIK3CD |
| CD40LG ELISA Kit | publications with ICOS and CD40LG |
| PIK3CB ELISA Kit | publications with ICOS and PIK3CB |
| PIK3CG ELISA Kit | publications with ICOS and PIK3CG |
Pathways associated with ICOS ELISA Kit
| Adaptive Immune System Pathway | Adaptive Immune System Pathway |
| Cell Adhesion Molecules (CAMs) Pathway | Cell Adhesion Molecules (CAMs) Pathway |
| Costimulation By The CD28 Family Pathway | Costimulation By The CD28 Family Pathway |
| Immune System Pathway | Immune System Pathway |
| Intestinal Immune Network For IgA Production Pathway | Intestinal Immune Network For IgA Production Pathway |
| Primary Immunodeficiency Pathway | Primary Immunodeficiency Pathway |
| T Cell Receptor Signaling Pathway | T Cell Receptor Signaling Pathway |
Diseases associated with ICOS ELISA Kit
| Inflammation | publications with ICOS and Inflammation |
| Death | publications with ICOS and Death |
| Disease Models, Animal | publications with ICOS and Disease Models, Animal |
| Necrosis | publications with ICOS and Necrosis |
| Respiratory Tract Diseases | publications with ICOS and Respiratory Tract Diseases |
| Hypersensitivity | publications with ICOS and Hypersensitivity |
| Lung Diseases | publications with ICOS and Lung Diseases |
| Common Variable Immunodeficiency | publications with ICOS and Common Variable Immunodeficiency |
| Respiratory Hypersensitivity | publications with ICOS and Respiratory Hypersensitivity |
| Liver Diseases | publications with ICOS and Liver Diseases |
Organs/Tissues associated with ICOS ELISA Kit
| Blood | publications with ICOS and Blood |
| Lung | publications with ICOS and Lung |
| Heart | publications with ICOS and Heart |
| Brain | publications with ICOS and Brain |
| Thymus | publications with ICOS and Thymus |
| Pharynx | publications with ICOS and Pharynx |
| Thyroid | publications with ICOS and Thyroid |
| Uterus | publications with ICOS and Uterus |
