KEY FEATURES
| Full Name | Interleukin 2 Receptor Gamma |
|---|---|
| Synonym | CD132; IL2R-g; IL2-RG; CD132; IMD4; p64; SCIDX; SCIDX1; Cytokine Receptor Common Gamma Chain; Severe Combined Immunodeficiency |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.15-10ng/mL |
| Sensitivity | 0.06ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human IL-2Rγ in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-2Rγ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-2Rγ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-2Rγ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-2Rγ. You can calculate the concentration of Human IL-2Rγ in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human IL-2Rγ ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.51 | 1.25 | 3.86 | 0.51 | 1.34 | 4.11 |
| Standard deviation | 0.03 | 0.06 | 0.15 | 0.03 | 0.07 | 0.17 |
| CV(%) | 6.28 | 4.6 | 3.87 | 5.92 | 5.04 | 4.19 |
RECOVERY
The recovery of Human IL-2Rγ spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-108 | 102 |
| EDTA plasma (n=8) | 85-95 | 90 |
| Cell culture media (n=8) | 89-100 | 95 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human IL-2Rγ in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Pathways associated with ELISA Kit
| Amine-derived Hormones Pathway | Amine-derived Hormones Pathway |
| Autoimmune Thyroid Disease Pathway | Autoimmune Thyroid Disease Pathway |
| Cytokine-cytokine Receptor Interaction Pathway | Cytokine-cytokine Receptor Interaction Pathway |
| Hematopoietic Cell Lineage Pathway | Hematopoietic Cell Lineage Pathway |
| Jak-STAT Signaling Pathway | Jak-STAT Signaling Pathway |
| Metabolism Pathway | Metabolism Pathway |
Diseases associated with TPO ELISA Kit
| Thyroid Diseases | publications with TPO and Thyroid Diseases |
| Hypothyroidism | publications with TPO and Hypothyroidism |
| Goiter | publications with TPO and Goiter |
| Thyroid Neoplasms | publications with TPO and Thyroid Neoplasms |
| Carcinoma | publications with TPO and Carcinoma |
| Blood Coagulation Disorders | publications with TPO and Blood Coagulation Disorders |
| Liver Diseases | publications with TPO and Liver Diseases |
| Congenital Hypothyroidism | publications with TPO and Congenital Hypothyroidism |
| Hepatitis | publications with TPO and Hepatitis |
| Mental Disorders | publications with TPO and Mental Disorders |
Organs/Tissues associated with TPO ELISA Kit
| Blood | publications with TPO and Blood |
| Thyroid | publications with TPO and Thyroid |
| Brain | publications with TPO and Brain |
| Spleen | publications with TPO and Spleen |
| Muscle | publications with TPO and Muscle |
| Heart | publications with TPO and Heart |
| Connective Tissue | publications with TPO and Connective Tissue |
| Intestine | publications with TPO and Intestine |
| Adipose Tissue | publications with TPO and Adipose Tissue |
| Testis | publications with TPO and Testis |
