KEY FEATURES
| Full Name | Interleukin 1 Receptor Associated Kinase 1 |
|---|---|
| Synonym | IRAK1; IRAK; Pelle |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.31-20ng/mL |
| Sensitivity | 0.12ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human IRAK1 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IRAK1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IRAK1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IRAK1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IRAK1. You can calculate the concentration of Human IRAK1 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human IRAK1 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.98 | 3.06 | 7.43 | 0.91 | 2.78 | 7.46 |
| Standard deviation | 0.06 | 0.16 | 0.36 | 0.05 | 0.14 | 0.27 |
| CV(%) | 5.66 | 5.09 | 4.83 | 5.57 | 5.04 | 3.65 |
RECOVERY
The recovery of Human IRAK1 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 86-101 | 92 |
| EDTA plasma (n=8) | 86-98 | 91 |
| Cell culture media (n=8) | 86-99 | 92 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human IRAK1 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with IRAK ELISA Kits
| TRAF6 ELISA Kit | publications with IRAK and TRAF6 |
| MYD88 ELISA Kit | publications with IRAK and MYD88 |
| TLR4 ELISA Kit | publications with IRAK and TLR4 |
| IRAK4 ELISA Kit | publications with IRAK and IRAK4 |
| TOLLIP ELISA Kit | publications with IRAK and TOLLIP |
| IRF7 ELISA Kit | publications with IRAK and IRF7 |
| PELI1 ELISA Kit | publications with IRAK and PELI1 |
| PELI2 ELISA Kit | publications with IRAK and PELI2 |
| UBC ELISA Kit | publications with IRAK and UBC |
| PELI3 ELISA Kit | publications with IRAK and PELI3 |
Pathways associated with IRAK ELISA Kit
| Activated TLR4 Signalling Pathway | Activated TLR4 Signalling Pathway |
| Apoptosis Pathway | Apoptosis Pathway |
| Apoptosis Modulation And Signaling Pathway | Apoptosis Modulation And Signaling Pathway |
| Chagas Disease (American Trypanosomiasis) Pathway | Chagas Disease (American Trypanosomiasis) Pathway |
| Cytokine Signaling In Immune System Pathway | Cytokine Signaling In Immune System Pathway |
| EBV LMP1 Signaling Pathway | EBV LMP1 Signaling Pathway |
| Epstein-Barr Virus Infection Pathway | Epstein-Barr Virus Infection Pathway |
Diseases associated with IRAK ELISA Kit
| Inflammation | publications with IRAK and Inflammation |
| Necrosis | publications with IRAK and Necrosis |
| Neoplasms | publications with IRAK and Neoplasms |
| Cardiovascular Diseases | publications with IRAK and Cardiovascular Diseases |
| Arthritis, Rheumatoid | publications with IRAK and Arthritis, Rheumatoid |
| Disease Models, Animal | publications with IRAK and Disease Models, Animal |
| Lung Diseases | publications with IRAK and Lung Diseases |
| Liver Diseases | publications with IRAK and Liver Diseases |
| Drug Toxicity | publications with IRAK and Drug Toxicity |
| Adenocarcinoma | publications with IRAK and Adenocarcinoma |
Organs/Tissues associated with IRAK ELISA Kit
| Blood | publications with IRAK and Blood |
| Liver | publications with IRAK and Liver |
| Bone | publications with IRAK and Bone |
| Lung | publications with IRAK and Lung |
| Kidney | publications with IRAK and Kidney |
| Brain | publications with IRAK and Brain |
| Vascular | publications with IRAK and Vascular |
| Bone Marrow | publications with IRAK and Bone Marrow |
| Intestine | publications with IRAK and Intestine |
| Heart | publications with IRAK and Heart |
