KEY FEATURES
| Full Name | Lymphotoxin Beta Receptor |
|---|---|
| Synonym | TNFCR; TNFR-RP; TNFR-III; TNFR2-RP; TNFRSF3; Tumor Necrosis Factor Superfamily Member 3; Tumor necrosis factor C receptor; Tumor necrosis factor receptor type III |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.31-20ng/mL |
| Sensitivity | 0.12ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human LTbR in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LTbR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LTbR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LTbR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LTbR. You can calculate the concentration of Human LTbR in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human LTbR ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.91 | 2.56 | 6.92 | 0.93 | 2.67 | 6.37 |
| Standard deviation | 0.06 | 0.14 | 0.22 | 0.05 | 0.14 | 0.22 |
| CV(%) | 6.29 | 5.46 | 3.11 | 5.62 | 5.11 | 3.5 |
RECOVERY
The recovery of Human LTbR spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-105 | 99 |
| EDTA plasma (n=8) | 89-105 | 96 |
| Cell culture media (n=8) | 88-101 | 94 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human LTbR in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with LTbR ELISA Kits
| TNFSF14 ELISA Kit | publications with LTbR and TNFSF14 |
| TRAF3 ELISA Kit | publications with LTbR and TRAF3 |
| TRAF2 ELISA Kit | publications with LTbR and TRAF2 |
| TRAF5 ELISA Kit | publications with LTbR and TRAF5 |
| MADCAM1 ELISA Kit | publications with LTbR and MADCAM1 |
| MAP3K14 ELISA Kit | publications with LTbR and MAP3K14 |
Pathways associated with LTbR ELISA Kit
| Cytokine-cytokine Receptor Interaction Pathway | Cytokine-cytokine Receptor Interaction Pathway |
| HIF-1 Signaling Pathway | HIF-1 Signaling Pathway |
| HTLV-I Infection Pathway | HTLV-I Infection Pathway |
| Intestinal Immune Network For IgA Production Pathway | Intestinal Immune Network For IgA Production Pathway |
| NF-kappa B Signaling Pathway | NF-kappa B Signaling Pathway |
| Viral Carcinogenesis Pathway | Viral Carcinogenesis Pathway |
Diseases associated with LTbR ELISA Kit
| Necrosis | publications with LTbR and Necrosis |
| Inflammation | publications with LTbR and Inflammation |
| Disease Models, Animal | publications with LTbR and Disease Models, Animal |
| Nervous System Diseases | publications with LTbR and Nervous System Diseases |
| Neoplasms, Experimental | publications with LTbR and Neoplasms, Experimental |
| Liver Diseases | publications with LTbR and Liver Diseases |
| Breast Neoplasms | publications with LTbR and Breast Neoplasms |
| Liver Neoplasms | publications with LTbR and Liver Neoplasms |
| Weight Loss | publications with LTbR and Weight Loss |
| Prostatic Diseases | publications with LTbR and Prostatic Diseases |
Organs/Tissues associated with LTbR ELISA Kit
| Blood | publications with LTbR and Blood |
| Lymph Node | publications with LTbR and Lymph Node |
| Spleen | publications with LTbR and Spleen |
| Bone | publications with LTbR and Bone |
| Bone Marrow | publications with LTbR and Bone Marrow |
| Intestine | publications with LTbR and Intestine |
| Embryonic Tissue | publications with LTbR and Embryonic Tissue |
| Thymus | publications with LTbR and Thymus |
| Vascular | publications with LTbR and Vascular |
| Liver | publications with LTbR and Liver |
