KEY FEATURES
| Full Name | Myeloid Progenitor Inhibitory Factor 1 |
|---|---|
| Synonym | CCL23; SCYA23; Ckb-8; MIP-3; CKb8; Chemokine(C-C-Motif)ligand 23; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 23; Macrophage inflammatory protein 3 |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 15.6-1000pg/mL |
| Sensitivity | 6pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human MPIF1 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MPIF1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MPIF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MPIF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MPIF1. You can calculate the concentration of Human MPIF1 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human MPIF1 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 48.88 | 143.97 | 407.11 | 46.49 | 144.27 | 371.04 |
| Standard deviation | 2.54 | 6.92 | 19.74 | 3.17 | 6.95 | 19.59 |
| CV(%) | 5.2 | 4.81 | 4.85 | 6.82 | 4.82 | 5.28 |
RECOVERY
The recovery of Human MPIF1 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 87-98 | 93 |
| EDTA plasma (n=8) | 89-100 | 94 |
| Cell culture media (n=8) | 83-98 | 90 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human MPIF1 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with CCL23 ELISA Kits
| CCR1 ELISA Kit | publications with CCL23 and CCR1 |
| CCR2 ELISA Kit | publications with CCL23 and CCR2 |
| CCR3 ELISA Kit | publications with CCL23 and CCR3 |
| CXCR4 ELISA Kit | publications with CCL23 and CXCR4 |
| CCR5 ELISA Kit | publications with CCL23 and CCR5 |
| CX3CR1 ELISA Kit | publications with CCL23 and CX3CR1 |
Pathways associated with CCL23 ELISA Kit
| Chemokine Signaling Pathway | Chemokine Signaling Pathway |
| Class A/1 (Rhodopsin-like Receptors) Pathway | Class A/1 (Rhodopsin-like Receptors) Pathway |
| Cytokine-cytokine Receptor Interaction Pathway | Cytokine-cytokine Receptor Interaction Pathway |
| Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway | Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway |
| G Alpha (i) Signalling Events Pathway | G Alpha (i) Signalling Events Pathway |
| G Alpha (q) Signalling Events Pathway | G Alpha (q) Signalling Events Pathway |
| GPCR Downstream Signaling Pathway | GPCR Downstream Signaling Pathway |
| GPCR Ligand Binding Pathway | GPCR Ligand Binding Pathway |
Diseases associated with CCL23 ELISA Kit
| Carcinoma | publications with CCL23 and Carcinoma |
| Cardiovascular Diseases | publications with CCL23 and Cardiovascular Diseases |
| Premature Birth | publications with CCL23 and Premature Birth |
| Obstetric Labor, Premature | publications with CCL23 and Obstetric Labor, Premature |
| Leukemia, Myeloid, Acute | publications with CCL23 and Leukemia, Myeloid, Acute |
| Heart Diseases | publications with CCL23 and Heart Diseases |
| Fetal Resorption | publications with CCL23 and Fetal Resorption |
| Bone Neoplasms | publications with CCL23 and Bone Neoplasms |
| Fatty Liver | publications with CCL23 and Fatty Liver |
Organs/Tissues associated with CCL23 ELISA Kit
| Placenta | publications with CCL23 and Placenta |
| Liver | publications with CCL23 and Liver |
| Lung | publications with CCL23 and Lung |
| Uncharacterized Tissue | publications with CCL23 and Uncharacterized Tissue |
