KEY FEATURES
| Full Name | Nicotinamide Adenine Dinucleotide Phosphate Oxidase 5 |
|---|---|
| Synonym | NOX5;NADPH oxidase 5; |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 31.25-2000pg/mL |
| Sensitivity | 18.75pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human NOX5 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NOX5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NOX5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOX5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NOX5. You can calculate the concentration of Human NOX5 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human NOX5 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 96.74 | 282.6 | 984.71 | 88.79 | 259.73 | 957.07 |
| Standard deviation | 6.6 | 16.45 | 53.08 | 6.04 | 14 | 43.36 |
| CV(%) | 6.82 | 5.82 | 5.39 | 6.8 | 5.39 | 4.53 |
RECOVERY
The recovery of Human NOX5 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 93-104 | 99 |
| EDTA plasma (n=8) | 95-106 | 101 |
| Cell culture media (n=8) | 86-100 | 92 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human NOX5 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with NOX5 ELISA Kits
| NOXA1 ELISA Kit | publications with NOX5 and NOXA1 |
| NOXO1 ELISA Kit | publications with NOX5 and NOXO1 |
| CYBA ELISA Kit | publications with NOX5 and CYBA |
| SUMO1 ELISA Kit | publications with NOX5 and SUMO1 |
| CD22 ELISA Kit | publications with NOX5 and CD22 |
| HVCN1 ELISA Kit | publications with NOX5 and HVCN1 |
Diseases associated with NOX5 ELISA Kit
| Cardiovascular Diseases | publications with NOX5 and Cardiovascular Diseases |
| Inflammation | publications with NOX5 and Inflammation |
| Hypertension | publications with NOX5 and Hypertension |
| Adenocarcinoma | publications with NOX5 and Adenocarcinoma |
| Necrosis | publications with NOX5 and Necrosis |
| Kidney Diseases | publications with NOX5 and Kidney Diseases |
| Heart Diseases | publications with NOX5 and Heart Diseases |
| Lung Diseases | publications with NOX5 and Lung Diseases |
| Heart Failure | publications with NOX5 and Heart Failure |
| Hyperglycemia | publications with NOX5 and Hyperglycemia |
Organs/Tissues associated with NOX5 ELISA Kit
| Vascular | publications with NOX5 and Vascular |
| Blood | publications with NOX5 and Blood |
| Kidney | publications with NOX5 and Kidney |
| Lung | publications with NOX5 and Lung |
| Prostate | publications with NOX5 and Prostate |
| Testis | publications with NOX5 and Testis |
| Skin | publications with NOX5 and Skin |
| Spleen | publications with NOX5 and Spleen |
| Brain | publications with NOX5 and Brain |
| Placenta | publications with NOX5 and Placenta |
