KEY FEATURES
| Full Name | Oncostatin M Receptor |
|---|---|
| Synonym | OSMRB; IL-31RB; Oncostatin-M-specific receptor subunit beta; Interleukin-31 receptor subunit beta |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.15-10ng/mL |
| Sensitivity | 0.07ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human OSMR in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OSMR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OSMR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OSMR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OSMR. You can calculate the concentration of Human OSMR in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human OSMR ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.48 | 1 | 3.55 | 0.43 | 1.04 | 3.38 |
| Standard deviation | 0.03 | 0.06 | 0.15 | 0.02 | 0.05 | 0.18 |
| CV(%) | 6.16 | 5.84 | 4.22 | 5.64 | 5.01 | 5.32 |
RECOVERY
The recovery of Human OSMR spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 85-97 | 92 |
| EDTA plasma (n=8) | 85-95 | 90 |
| Cell culture media (n=8) | 91-104 | 98 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human OSMR in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with OSMR ELISA Kits
| OSM ELISA Kit | publications with OSMR and OSM |
| IL6ST ELISA Kit | publications with OSMR and IL6ST |
| JAK1 ELISA Kit | publications with OSMR and JAK1 |
| JAK2 ELISA Kit | publications with OSMR and JAK2 |
| SOCS3 ELISA Kit | publications with OSMR and SOCS3 |
| SOCS1 ELISA Kit | publications with OSMR and SOCS1 |
| JAK3 ELISA Kit | publications with OSMR and JAK3 |
| TYK2 ELISA Kit | publications with OSMR and TYK2 |
Pathways associated with OSMR ELISA Kit
| Cytokine-cytokine Receptor Interaction Pathway | Cytokine-cytokine Receptor Interaction Pathway |
| Jak-STAT Signaling Pathway | Jak-STAT Signaling Pathway |
| Oncostatin M Signaling Pathway | Oncostatin M Signaling Pathway |
| PI3K-Akt Signaling Pathway | PI3K-Akt Signaling Pathway |
Diseases associated with OSMR ELISA Kit
| Inflammation | publications with OSMR and Inflammation |
| Carcinoma | publications with OSMR and Carcinoma |
| Liver Diseases | publications with OSMR and Liver Diseases |
| Necrosis | publications with OSMR and Necrosis |
| Breast Neoplasms | publications with OSMR and Breast Neoplasms |
| Lung Neoplasms | publications with OSMR and Lung Neoplasms |
| Liver Neoplasms | publications with OSMR and Liver Neoplasms |
| Carcinoma, Hepatocellular | publications with OSMR and Carcinoma, Hepatocellular |
| Drug-Induced Liver Injury | publications with OSMR and Drug-Induced Liver Injury |
| Lung Diseases | publications with OSMR and Lung Diseases |
Organs/Tissues associated with OSMR ELISA Kit
| Skin | publications with OSMR and Skin |
| Bone | publications with OSMR and Bone |
| Liver | publications with OSMR and Liver |
| Lung | publications with OSMR and Lung |
| Vascular | publications with OSMR and Vascular |
| Kidney | publications with OSMR and Kidney |
| Brain | publications with OSMR and Brain |
| Intestine | publications with OSMR and Intestine |
| Nerve | publications with OSMR and Nerve |
| Embryonic Tissue | publications with OSMR and Embryonic Tissue |
