KEY FEATURES
| Full Name | Ornithine Transcarbamylase |
|---|---|
| Synonym | OCTD; OCT; OTCase; Ornithine Transcarbamylase; Ornithine Carbamoyltransferase |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 3.12-200ng/mL |
| Sensitivity | 1.24ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human OTC in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OTC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OTC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OTC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OTC. You can calculate the concentration of Human OTC in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human OTC ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 10.55 | 21.75 | 70.01 | 10.31 | 23.03 | 70.27 |
| Standard deviation | 0.64 | 0.91 | 2.7 | 0.6 | 1.13 | 3.53 |
| CV(%) | 6.11 | 4.19 | 3.85 | 5.86 | 4.9 | 5.02 |
RECOVERY
The recovery of Human OTC spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 88-104 | 95 |
| EDTA plasma (n=8) | 88-105 | 96 |
| Cell culture media (n=8) | 91-105 | 96 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human OTC in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with OTC ELISA Kits
| CPS1 ELISA Kit | publications with OTC and CPS1 |
| ARG1 ELISA Kit | publications with OTC and ARG1 |
| ASS1 ELISA Kit | publications with OTC and ASS1 |
| OAT ELISA Kit | publications with OTC and OAT |
| ARG2 ELISA Kit | publications with OTC and ARG2 |
| ACY1 ELISA Kit | publications with OTC and ACY1 |
| CAD ELISA Kit | publications with OTC and CAD |
Pathways associated with OTC ELISA Kit
| Arginine And Proline Metabolism Pathway | Arginine And Proline Metabolism Pathway |
| Biosynthesis Of Amino Acids Pathway | Biosynthesis Of Amino Acids Pathway |
| Metabolic Pathways | Metabolic Pathways |
| Metabolism Pathway | Metabolism Pathway |
| Metabolism Of Amino Acids And Derivatives Pathway | Metabolism Of Amino Acids And Derivatives Pathway |
| Urea Cycle Pathway | Urea Cycle Pathway |
Diseases associated with OTC ELISA Kit
| Brain Diseases | publications with OTC and Brain Diseases |
| ORNITHINE TRANSCARBAMYLASE DEFICIENCY, HYPERAMMONEMIA DUE TO | publications with OTC and ORNITHINE TRANSCARBAMYLASE DEFICIENCY, HYPERAMMONEMIA DUE TO |
| Ornithine Carbamoyltransferase Deficiency Disease | publications with OTC and Ornithine Carbamoyltransferase Deficiency Disease |
| Hyperammonemia | publications with OTC and Hyperammonemia |
| Gastrointestinal Diseases | publications with OTC and Gastrointestinal Diseases |
| Liver Diseases | publications with OTC and Liver Diseases |
| Drug-Related Side Effects and Adverse Reactions | publications with OTC and Drug-Related Side Effects and Adverse Reactions |
| Disease Models, Animal | publications with OTC and Disease Models, Animal |
| Heart Diseases | publications with OTC and Heart Diseases |
| Inflammation | publications with OTC and Inflammation |
Organs/Tissues associated with OTC ELISA Kit
| Liver | publications with OTC and Liver |
| Connective Tissue | publications with OTC and Connective Tissue |
| Embryonic Tissue | publications with OTC and Embryonic Tissue |
| Testis | publications with OTC and Testis |
