KEY FEATURES
| Full Name | Palladin |
|---|---|
| Synonym | Cytoskeletal Associated Protein; Sarcoma antigen NY-SAR-77 |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.15-10ng/mL |
| Sensitivity | 0.06ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human PALLD in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PALLD. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PALLD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PALLD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PALLD. You can calculate the concentration of Human PALLD in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human PALLD ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.5 | 1.36 | 4.71 | 0.52 | 1.4 | 4.8 |
| Standard deviation | 0.03 | 0.06 | 0.2 | 0.03 | 0.06 | 0.25 |
| CV(%) | 6.71 | 4.3 | 4.19 | 6.54 | 4.12 | 5.16 |
RECOVERY
The recovery of Human PALLD spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 91-102 | 96 |
| EDTA plasma (n=8) | 85-99 | 92 |
| Cell culture media (n=8) | 97-108 | 102 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human PALLD in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Pathways associated with ELISA Kit
| PodNet: Protein-protein Interactions In The Podocyte Pathway | PodNet: Protein-protein Interactions In The Podocyte Pathway |
| XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway | XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway |
Diseases associated with PALLD ELISA Kit
| Pancreatic Neoplasms | publications with PALLD and Pancreatic Neoplasms |
| Heart Diseases | publications with PALLD and Heart Diseases |
| Nervous System Diseases | publications with PALLD and Nervous System Diseases |
| Breast Neoplasms | publications with PALLD and Breast Neoplasms |
| Melanoma | publications with PALLD and Melanoma |
| Myocardial Infarction | publications with PALLD and Myocardial Infarction |
| Hypertension | publications with PALLD and Hypertension |
| Precancerous Conditions | publications with PALLD and Precancerous Conditions |
| Fatty Liver | publications with PALLD and Fatty Liver |
| Liver Diseases | publications with PALLD and Liver Diseases |
Organs/Tissues associated with PALLD ELISA Kit
| Blood | publications with PALLD and Blood |
| Liver | publications with PALLD and Liver |
| Testis | publications with PALLD and Testis |
| Heart | publications with PALLD and Heart |
| Connective Tissue | publications with PALLD and Connective Tissue |
| Lung | publications with PALLD and Lung |
| Skin | publications with PALLD and Skin |
| Pancreas | publications with PALLD and Pancreas |
| Prostate | publications with PALLD and Prostate |
| Embryonic Tissue | publications with PALLD and Embryonic Tissue |
