KEY FEATURES
| Full Name | Platelet Derived Growth Factor C |
|---|---|
| Synonym | PDGF-C; SCDGF; VEGF-E; Fallotein; Spinal cord-derived growth factor; Platelet-derived growth factor C, latent form; Platelet-derived growth factor C, receptor-binding form |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 125-8000pg/mL |
| Sensitivity | 43pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human PDGFC in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDGFC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDGFC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDGFC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PDGFC. You can calculate the concentration of Human PDGFC in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human PDGFC ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 417.61 | 736.87 | 3155.36 | 411.31 | 694.57 | 3169.28 |
| Standard deviation | 23.18 | 40.75 | 101.29 | 22.79 | 29.31 | 174.31 |
| CV(%) | 5.55 | 5.53 | 3.21 | 5.54 | 4.22 | 5.5 |
RECOVERY
The recovery of Human PDGFC spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 85-99 | 92 |
| EDTA plasma (n=8) | 89-102 | 96 |
| Cell culture media (n=8) | 93-104 | 98 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human PDGFC in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with PDGFC ELISA Kits
| PDGFRA ELISA Kit | publications with PDGFC and PDGFRA |
| PDGFRB ELISA Kit | publications with PDGFC and PDGFRB |
| FGFR2 ELISA Kit | publications with PDGFC and FGFR2 |
| FGFR1 ELISA Kit | publications with PDGFC and FGFR1 |
| FGF2 ELISA Kit | publications with PDGFC and FGF2 |
Pathways associated with PDGFC ELISA Kit
| Choline Metabolism In Cancer Pathway | Choline Metabolism In Cancer Pathway |
| Cytokine-cytokine Receptor Interaction Pathway | Cytokine-cytokine Receptor Interaction Pathway |
| Focal Adhesion Pathway | Focal Adhesion Pathway |
| Gap Junction Pathway | Gap Junction Pathway |
| Melanoma Pathway | Melanoma Pathway |
Diseases associated with PDGFC ELISA Kit
| Cleft Palate | publications with PDGFC and Cleft Palate |
| Congenital Abnormalities | publications with PDGFC and Congenital Abnormalities |
| Breast Neoplasms | publications with PDGFC and Breast Neoplasms |
| Cleft Lip | publications with PDGFC and Cleft Lip |
| Lung Neoplasms | publications with PDGFC and Lung Neoplasms |
| Neoplasms, Experimental | publications with PDGFC and Neoplasms, Experimental |
| Liver Diseases | publications with PDGFC and Liver Diseases |
| Liver Neoplasms | publications with PDGFC and Liver Neoplasms |
| Carcinoma, Hepatocellular | publications with PDGFC and Carcinoma, Hepatocellular |
| Disease Models, Animal | publications with PDGFC and Disease Models, Animal |
Organs/Tissues associated with PDGFC ELISA Kit
| Vascular | publications with PDGFC and Vascular |
| Blood | publications with PDGFC and Blood |
| Lung | publications with PDGFC and Lung |
| Kidney | publications with PDGFC and Kidney |
| Embryonic Tissue | publications with PDGFC and Embryonic Tissue |
| Skin | publications with PDGFC and Skin |
| Liver | publications with PDGFC and Liver |
| Mouth | publications with PDGFC and Mouth |
| Uterus | publications with PDGFC and Uterus |
| Ovary | publications with PDGFC and Ovary |
