KEY FEATURES
| Full Name | Phosphofructokinase, Platelet |
|---|---|
| Synonym | PFK-C; PFKF; ATP-dependent 6-phosphofructokinase, platelet type; 6-phosphofructokinase type C; Phosphofructo-1-kinase isozyme C; Phosphohexokinase |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.31-20ng/mL |
| Sensitivity | 0.12ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human PFKP in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PFKP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PFKP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PFKP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PFKP. You can calculate the concentration of Human PFKP in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human PFKP ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.97 | 2.64 | 7.52 | 0.93 | 2.62 | 7.5 |
| Standard deviation | 0.05 | 0.13 | 0.3 | 0.05 | 0.15 | 0.32 |
| CV(%) | 5.32 | 4.96 | 3.97 | 5.73 | 5.73 | 4.32 |
RECOVERY
The recovery of Human PFKP spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-110 | 102 |
| EDTA plasma (n=8) | 91-107 | 99 |
| Cell culture media (n=8) | 93-109 | 101 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human PFKP in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with PFKP ELISA Kits
| ALDOA ELISA Kit | publications with PFKP and ALDOA |
| HK2 ELISA Kit | publications with PFKP and HK2 |
| HK1 ELISA Kit | publications with PFKP and HK1 |
| PFKFB3 ELISA Kit | publications with PFKP and PFKFB3 |
| GPI ELISA Kit | publications with PFKP and GPI |
Pathways associated with PFKP ELISA Kit
| AMPK Signaling Pathway | AMPK Signaling Pathway |
| Biosynthesis Of Amino Acids Pathway | Biosynthesis Of Amino Acids Pathway |
| Carbon Metabolism Pathway | Carbon Metabolism Pathway |
| Central Carbon Metabolism In Cancer Pathway | Central Carbon Metabolism In Cancer Pathway |
| Disease Pathway | Disease Pathway |
| Fructose And Mannose Metabolism Pathway | Fructose And Mannose Metabolism Pathway |
Diseases associated with PFKP ELISA Kit
| Disease Models, Animal | publications with PFKP and Disease Models, Animal |
| Lung Neoplasms | publications with PFKP and Lung Neoplasms |
| Hypertension | publications with PFKP and Hypertension |
| Weight Gain | publications with PFKP and Weight Gain |
| Neoplasms, Experimental | publications with PFKP and Neoplasms, Experimental |
| Breast Neoplasms | publications with PFKP and Breast Neoplasms |
| Carcinoma, Hepatocellular | publications with PFKP and Carcinoma, Hepatocellular |
| Liver Neoplasms | publications with PFKP and Liver Neoplasms |
| Adenocarcinoma | publications with PFKP and Adenocarcinoma |
| Liver Diseases | publications with PFKP and Liver Diseases |
Organs/Tissues associated with PFKP ELISA Kit
| Ovary | publications with PFKP and Ovary |
| Blood | publications with PFKP and Blood |
| Liver | publications with PFKP and Liver |
| Muscle | publications with PFKP and Muscle |
| Bone | publications with PFKP and Bone |
| Thyroid | publications with PFKP and Thyroid |
| Brain | publications with PFKP and Brain |
| Vascular | publications with PFKP and Vascular |
| Skin | publications with PFKP and Skin |
| Kidney | publications with PFKP and Kidney |
