KEY FEATURES
| Full Name | S100 Calcium Binding Protein A10 |
|---|---|
| Synonym | ANX2L; ANX2LG; CAL1L; CLP11; GP11; P11; P10; Calpactin-1 light chain; Cellular ligand of annexin II; Annexin II Ligand,Calpactin I,Light Polypeptide |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.15-10ng/mL |
| Sensitivity | 0.06ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human S100A10 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human S100A10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human S100A10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100A10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human S100A10. You can calculate the concentration of Human S100A10 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human S100A10 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.47 | 1.3 | 4.62 | 0.51 | 1.26 | 4.69 |
| Standard deviation | 0.02 | 0.05 | 0.2 | 0.03 | 0.05 | 0.21 |
| CV(%) | 5.05 | 4.05 | 4.4 | 5.58 | 4.12 | 4.47 |
RECOVERY
The recovery of Human S100A10 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 88-101 | 94 |
| EDTA plasma (n=8) | 90-101 | 95 |
| Cell culture media (n=8) | 88-103 | 94 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human S100A10 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with S100a10 ELISA Kits
| Anxa2 ELISA Kit | publications with S100a10 and Anxa2 |
| S100a6 ELISA Kit | publications with S100a10 and S100a6 |
| Cftr ELISA Kit | publications with S100a10 and Cftr |
| Anxa1 ELISA Kit | publications with S100a10 and Anxa1 |
| Plg ELISA Kit | publications with S100a10 and Plg |
| Trpv6 ELISA Kit | publications with S100a10 and Trpv6 |
| Dlc1 ELISA Kit | publications with S100a10 and Dlc1 |
Pathways associated with S100a10 ELISA Kit
| Dissolution Of Fibrin Clot Pathway | Dissolution Of Fibrin Clot Pathway |
| Hemostasis Pathway | Hemostasis Pathway |
| Prostaglandin Synthesis And Regulation Pathway | Prostaglandin Synthesis And Regulation Pathway |
Diseases associated with S100a10 ELISA Kit
| Inflammation | publications with S100a10 and Inflammation |
| Carcinoma | publications with S100a10 and Carcinoma |
| Disease Models, Animal | publications with S100a10 and Disease Models, Animal |
| Necrosis | publications with S100a10 and Necrosis |
| Nervous System Diseases | publications with S100a10 and Nervous System Diseases |
| Brain Diseases | publications with S100a10 and Brain Diseases |
| Depressive Disorder | publications with S100a10 and Depressive Disorder |
| Breast Neoplasms | publications with S100a10 and Breast Neoplasms |
| Leukemia, Myeloid, Acute | publications with S100a10 and Leukemia, Myeloid, Acute |
| Neoplasm Metastasis | publications with S100a10 and Neoplasm Metastasis |
Organs/Tissues associated with S100a10 ELISA Kit
| Brain | publications with S100a10 and Brain |
| Blood | publications with S100a10 and Blood |
| Skin | publications with S100a10 and Skin |
| Kidney | publications with S100a10 and Kidney |
| Muscle | publications with S100a10 and Muscle |
| Lung | publications with S100a10 and Lung |
| Intestine | publications with S100a10 and Intestine |
| Liver | publications with S100a10 and Liver |
| Connective Tissue | publications with S100a10 and Connective Tissue |
| Ovary | publications with S100a10 and Ovary |
