KEY FEATURES
| Full Name | Superoxide Dismutase 1 |
|---|---|
| Synonym | ALS; ALS1; IPOA; Superoxide Dismutase 1, Soluble; Amyotrophic Lateral Sclerosis 1,Adult; Superoxide dismutase [Cu-Zn] |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 62.5-4000pg/mL |
| Sensitivity | 25.1pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human SOD1 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SOD1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SOD1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SOD1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SOD1. You can calculate the concentration of Human SOD1 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human SOD1 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 182.7 | 619.7 | 1975.25 | 198.14 | 623.32 | 1970.54 |
| Standard deviation | 10.23 | 33.71 | 78.22 | 10.88 | 35.4 | 77.05 |
| CV(%) | 5.6 | 5.44 | 3.96 | 5.49 | 5.68 | 3.91 |
RECOVERY
The recovery of Human SOD1 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-110 | 101 |
| EDTA plasma (n=8) | 88-104 | 96 |
| Cell culture media (n=8) | 87-101 | 93 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human SOD1 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with SOD ELISA Kits
| SOD2 ELISA Kit | publications with SOD and SOD2 |
| CAT ELISA Kit | publications with SOD and CAT |
| CCS ELISA Kit | publications with SOD and CCS |
| BCL2 ELISA Kit | publications with SOD and BCL2 |
| RNF19A ELISA Kit | publications with SOD and RNF19A |
| UBC ELISA Kit | publications with SOD and UBC |
| DNAJB1 ELISA Kit | publications with SOD and DNAJB1 |
| ESR1 ELISA Kit | publications with SOD and ESR1 |
Pathways associated with SOD ELISA Kit
| AGE/RAGE Pathway | AGE/RAGE Pathway |
| Amyotrophic Lateral Sclerosis (ALS) Pathway | Amyotrophic Lateral Sclerosis (ALS) Pathway |
| Cellular Responses To Stress Pathway | Cellular Responses To Stress Pathway |
| Detoxification Of Reactive Oxygen Species Pathway | Detoxification Of Reactive Oxygen Species Pathway |
| Dopamine Metabolism Pathway | Dopamine Metabolism Pathway |
| FOXA1 Transcription Factor Network Pathway | FOXA1 Transcription Factor Network Pathway |
| Folate Metabolism Pathway | Folate Metabolism Pathway |
| Hemostasis Pathway | Hemostasis Pathway |
Diseases associated with SOD ELISA Kit
| Amyotrophic Lateral Sclerosis | publications with SOD and Amyotrophic Lateral Sclerosis |
| Nervous System Diseases | publications with SOD and Nervous System Diseases |
| Motor Neuron Disease | publications with SOD and Motor Neuron Disease |
| Disease Models, Animal | publications with SOD and Disease Models, Animal |
| Nerve Degeneration | publications with SOD and Nerve Degeneration |
| Protein Deficiency | publications with SOD and Protein Deficiency |
| Inflammation | publications with SOD and Inflammation |
| Ischemia | publications with SOD and Ischemia |
| Necrosis | publications with SOD and Necrosis |
| Brain Ischemia | publications with SOD and Brain Ischemia |
Organs/Tissues associated with SOD ELISA Kit
| Spinal Cord | publications with SOD and Spinal Cord |
| Brain | publications with SOD and Brain |
| Nerve | publications with SOD and Nerve |
| Muscle | publications with SOD and Muscle |
| Blood | publications with SOD and Blood |
| Liver | publications with SOD and Liver |
| Vascular | publications with SOD and Vascular |
| Kidney | publications with SOD and Kidney |
| Heart | publications with SOD and Heart |
| Bone | publications with SOD and Bone |
