KEY FEATURES
| Full Name | Toll Like Receptor 8 |
|---|---|
| Synonym | CD288 |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 0.31-20ng/mL |
| Sensitivity | 0.12ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human TLR8 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TLR8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TLR8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TLR8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TLR8. You can calculate the concentration of Human TLR8 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human TLR8 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.97 | 2.64 | 7.88 | 0.96 | 2.48 | 7.77 |
| Standard deviation | 0.06 | 0.13 | 0.36 | 0.05 | 0.13 | 0.33 |
| CV(%) | 6.39 | 4.85 | 4.56 | 5.7 | 5.06 | 4.27 |
RECOVERY
The recovery of Human TLR8 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 84-96 | 91 |
| EDTA plasma (n=8) | 83-98 | 90 |
| Cell culture media (n=8) | 87-101 | 94 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human TLR8 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with TLR8 ELISA Kits
| TLR7 ELISA Kit | publications with TLR8 and TLR7 |
| TLR9 ELISA Kit | publications with TLR8 and TLR9 |
| TLR3 ELISA Kit | publications with TLR8 and TLR3 |
| MYD88 ELISA Kit | publications with TLR8 and MYD88 |
| CD40 ELISA Kit | publications with TLR8 and CD40 |
| UNC93B1 ELISA Kit | publications with TLR8 and UNC93B1 |
Pathways associated with TLR8 ELISA Kit
| Immune System Pathway | Immune System Pathway |
| Innate Immune System Pathway | Innate Immune System Pathway |
| MyD88 Dependent Cascade Initiated On Endosome Pathway | MyD88 Dependent Cascade Initiated On Endosome Pathway |
| Regulation Of Toll-like Receptor Signaling Pathway | Regulation Of Toll-like Receptor Signaling Pathway |
| TRAF6 Mediated IRF7 Activation In TLR7/8 Or 9 Signaling Pathway | TRAF6 Mediated IRF7 Activation In TLR7/8 Or 9 Signaling Pathway |
| TRAF6 Mediated Induction Of NFkB And MAP Kinases Upon TLR7/8 Or 9 Activation Pathway | TRAF6 Mediated Induction Of NFkB And MAP Kinases Upon TLR7/8 Or 9 Activation Pathway |
| Toll Like Receptor 7/8 (TLR7/8) Cascade Pathway | Toll Like Receptor 7/8 (TLR7/8) Cascade Pathway |
| Toll Like Receptor 9 (TLR9) Cascade Pathway | Toll Like Receptor 9 (TLR9) Cascade Pathway |
| Toll-Like Receptors Cascades Pathway | Toll-Like Receptors Cascades Pathway |
| Toll-like Receptor Signaling Pathway | Toll-like Receptor Signaling Pathway |
Diseases associated with TLR8 ELISA Kit
| Inflammation | publications with TLR8 and Inflammation |
| Necrosis | publications with TLR8 and Necrosis |
| Skin Diseases | publications with TLR8 and Skin Diseases |
| Lung Diseases | publications with TLR8 and Lung Diseases |
| Death | publications with TLR8 and Death |
| Heart Diseases | publications with TLR8 and Heart Diseases |
| Skin Neoplasms | publications with TLR8 and Skin Neoplasms |
| Kidney Diseases | publications with TLR8 and Kidney Diseases |
| Fibrosis | publications with TLR8 and Fibrosis |
| Uterine Cervical Neoplasms | publications with TLR8 and Uterine Cervical Neoplasms |
Organs/Tissues associated with TLR8 ELISA Kit
| Blood | publications with TLR8 and Blood |
| Lung | publications with TLR8 and Lung |
| Spleen | publications with TLR8 and Spleen |
| Brain | publications with TLR8 and Brain |
| Kidney | publications with TLR8 and Kidney |
| Intestine | publications with TLR8 and Intestine |
| Vascular | publications with TLR8 and Vascular |
| Uterus | publications with TLR8 and Uterus |
| Placenta | publications with TLR8 and Placenta |
| Connective Tissue | publications with TLR8 and Connective Tissue |
