KEY FEATURES
| Full Name | VGF Nerve Growth Factor Inducible |
|---|---|
| Synonym | NERP1; NERP2; Neuro-Endocrine Specific Protein VGF; Neuroendocrine Regulatory Peptide |
| Assay Type | Sandwich |
| Reactivity | Human |
| Range | 78-5000pg/mL |
| Sensitivity | 37pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Human VGF in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VGF. You can calculate the concentration of Human VGF in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Human VGF ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 276.61 | 426.53 | 1875.94 | 298.74 | 444.68 | 2001.38 |
| Standard deviation | 18.48 | 20.9 | 75.6 | 20.64 | 25.7 | 99.07 |
| CV(%) | 6.68 | 4.9 | 4.03 | 6.91 | 5.78 | 4.95 |
RECOVERY
The recovery of Human VGF spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 84-98 | 90 |
| EDTA plasma (n=8) | 94-106 | 100 |
| Cell culture media (n=8) | 88-102 | 93 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human VGF in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with VGF ELISA Kits
| NGF ELISA Kit | publications with VGF and NGF |
| BDNF ELISA Kit | publications with VGF and BDNF |
| NPY ELISA Kit | publications with VGF and NPY |
| VIP ELISA Kit | publications with VGF and VIP |
Diseases associated with VGF ELISA Kit
| Nervous System Diseases | publications with VGF and Nervous System Diseases |
| Brain Diseases | publications with VGF and Brain Diseases |
| Disease Models, Animal | publications with VGF and Disease Models, Animal |
| Neurodegenerative Diseases | publications with VGF and Neurodegenerative Diseases |
| Gastrointestinal Diseases | publications with VGF and Gastrointestinal Diseases |
| Weight Loss | publications with VGF and Weight Loss |
| Inflammation | publications with VGF and Inflammation |
| Memory Disorders | publications with VGF and Memory Disorders |
| Seizures | publications with VGF and Seizures |
| Necrosis | publications with VGF and Necrosis |
Organs/Tissues associated with VGF ELISA Kit
| Brain | publications with VGF and Brain |
| Pituitary Gland | publications with VGF and Pituitary Gland |
| Pancreas | publications with VGF and Pancreas |
| Adrenal Gland | publications with VGF and Adrenal Gland |
| Ganglia | publications with VGF and Ganglia |
| Stomach | publications with VGF and Stomach |
| Embryonic Tissue | publications with VGF and Embryonic Tissue |
| Skin | publications with VGF and Skin |
| Eye | publications with VGF and Eye |
| Lung | publications with VGF and Lung |
