Product description
This ELISA kit is rapid, quantitative enzyme-linked immunosorbent assays (ELISA) for the determination of Malachite Green content in Food samples.
Key features
Assay Range: 0.025 ppb~0.4 ppb
Sensitivity: 0.025 ppb (ng/mL)
Species reactivity: Universal
Assay time: 75min
Result type: Quantitative
Category: Residues&contaminants
Kit type: ELISA
Detection type: Competitive
Precision: CV<10%
Recovery rate: 85%±15%
Specificity:
Malachite Green | 100% | ||||
Crystal violet | 80% | ||||
Leucomalachite green (oxidized) | 100% | ||||
Leucocrystal violet (oxidized) | 80% |
Detection limits:
Fish, shrimp | 0.1 ppb |
Storage and shelf life
The kit should be stored at 2~8°C and must not be frozen. The shelf life of this product is 1 year. Do not use the kit beyond the expiration date.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The kit consists of an enzyme-labeled plate pre-coated with coupled antigens, horseradish enzyme markers, antibodies, standard substances, and other supporting reagents. During detection, standard substances or sample solutions are added. Malachite Green in the samples competes with the pre-coated coupled antigens on the enzyme-labeled plate for Malachite Green specific antibody. After adding the enzyme markers, TMB substrate is used for color development. The absorbance value of the sample is negatively correlated with the content of Malachite Green it contains, and the residual amount of Malachite Green in the sample can be obtained by comparing with the standard curve.
Materials supplied
Item | Quantity | ||||
Assay Plate | 96T | ||||
Concentrated Standard (10 ppb) | 1 x 1 mL | ||||
HRP Conjugate | 1 x 11 mL | ||||
Antibody | 1 x 5.5 mL | ||||
TMB Substrate Reagent (A\B) | 2 x 6 mL | ||||
Stop Solution | 1 x 6 mL | ||||
Cosolvent | 1 x 6 mL | ||||
Oxidant | 1 x 6 mL | ||||
Wash Buffer (20×) | 1 x 40 mL | ||||
Reconstitution Buffer (10×) | 1 x 20 mL | ||||
Plate Sealer | 1 piece | ||||
Instruction Manual | 1 piece | ||||
Sealed Bag | 1 copy |
Standard curve reference
Standard | 1 | 2 | 3 | 4 | 5 | 6 | |||
Concentration (ppb=ng/mL=ng/g) | 0 | 0.025 | 0.05 | 0.1 | 0.2 | 0.4 | |||
OD Standard | 2.299 | 1.886 | 1.472 | 0.979 | 0.559 | 0.317 |
