KEY FEATURES
| Full Name | Activated Protein C |
|---|---|
| Synonym | Activated Protein C |
| Assay Type | Sandwich |
| Reactivity | Mouse |
| Range | 78-5000pg/mL |
| Sensitivity | 33pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse Activated Protein C in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Activated Protein C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Activated Protein C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Activated Protein C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Activated Protein C. You can calculate the concentration of Mouse Activated Protein C in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse Activated Protein C ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 272.02 | 432.14 | 1933.57 | 249.76 | 462.97 | 1758.4 |
| Standard deviation | 18.2 | 21.52 | 91.65 | 15.01 | 21.85 | 61.02 |
| CV(%) | 6.69 | 4.98 | 4.74 | 6.01 | 4.72 | 3.47 |
RECOVERY
The recovery of Mouse Activated Protein C spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 84-97 | 90 |
| EDTA plasma (n=8) | 88-100 | 94 |
| Cell culture media (n=8) | 92-103 | 98 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse Activated Protein C in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
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| F10 ELISA Kit | publications with FV and F10 |
| PROC ELISA Kit | publications with FV and PROC |
| PROS1 ELISA Kit | publications with FV and PROS1 |
Pathways associated with FV ELISA Kit
| Blood Clotting Cascade Pathway | Blood Clotting Cascade Pathway |
| Common Pathway | Common Pathway |
| Complement And Coagulation Cascades Pathway | Complement And Coagulation Cascades Pathway |
| Formation Of Fibrin Clot (Clotting Cascade) Pathway | Formation Of Fibrin Clot (Clotting Cascade) Pathway |
| Hemostasis Pathway | Hemostasis Pathway |
| Platelet Activation, Signaling And Aggregation Pathway | Platelet Activation, Signaling And Aggregation Pathway |
| Platelet Degranulation Pathway | Platelet Degranulation Pathway |
| Response To Elevated Platelet Cytosolic Ca2+ Pathway | Response To Elevated Platelet Cytosolic Ca2+ Pathway |
Diseases associated with FV ELISA Kit
| Cardiovascular Diseases | publications with FV and Cardiovascular Diseases |
| Thrombosis | publications with FV and Thrombosis |
| Blood Coagulation Disorders | publications with FV and Blood Coagulation Disorders |
| Inflammation | publications with FV and Inflammation |
| Venous Thrombosis | publications with FV and Venous Thrombosis |
| Thromboembolism | publications with FV and Thromboembolism |
| Liver Diseases | publications with FV and Liver Diseases |
| Venous Thromboembolism | publications with FV and Venous Thromboembolism |
| Necrosis | publications with FV and Necrosis |
| Stroke | publications with FV and Stroke |
Organs/Tissues associated with FV ELISA Kit
| Blood | publications with FV and Blood |
| Brain | publications with FV and Brain |
| Liver | publications with FV and Liver |
| Thymus | publications with FV and Thymus |
| Intestine | publications with FV and Intestine |
| Kidney | publications with FV and Kidney |
| Heart | publications with FV and Heart |
| Lung | publications with FV and Lung |
| Embryonic Tissue | publications with FV and Embryonic Tissue |
| Eye | publications with FV and Eye |
