KEY FEATURES
| Full Name | Bone Morphogenetic Protein 7 |
|---|---|
| Synonym | OP1; Osteogenic Protein-1; Eptotermin alfa |
| Assay Type | Sandwich |
| Reactivity | Mouse |
| Range | 15.6-1000pg/mL |
| Sensitivity | 6.1pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse BMP7 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse BMP7. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BMP7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BMP7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse BMP7. You can calculate the concentration of Mouse BMP7 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse BMP7 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 46.8 | 95.43 | 482.78 | 43.11 | 94.56 | 471.6 |
| Standard deviation | 3.04 | 4.68 | 15.35 | 2.55 | 5.18 | 25.42 |
| CV(%) | 6.5 | 4.9 | 3.18 | 5.91 | 5.48 | 5.39 |
RECOVERY
The recovery of Mouse BMP7 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 93-107 | 99 |
| EDTA plasma (n=8) | 87-100 | 93 |
| Cell culture media (n=8) | 93-109 | 101 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse BMP7 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with Bmp7 ELISA Kits
| Bmp2 ELISA Kit | publications with Bmp7 and Bmp2 |
| Bmp4 ELISA Kit | publications with Bmp7 and Bmp4 |
| Bmpr2 ELISA Kit | publications with Bmp7 and Bmpr2 |
| Bmpr1a ELISA Kit | publications with Bmp7 and Bmpr1a |
| Smad5 ELISA Kit | publications with Bmp7 and Smad5 |
| Bmpr1b ELISA Kit | publications with Bmp7 and Bmpr1b |
| Acvr1 ELISA Kit | publications with Bmp7 and Acvr1 |
| Smad7 ELISA Kit | publications with Bmp7 and Smad7 |
| Acvr2a ELISA Kit | publications with Bmp7 and Acvr2a |
| Nog ELISA Kit | publications with Bmp7 and Nog |
Pathways associated with Bmp7 ELISA Kit
| Axon Guidance Pathway | Axon Guidance Pathway |
| BMP Signaling Pathway | BMP Signaling Pathway |
| Cytokine-cytokine Receptor Interaction Pathway | Cytokine-cytokine Receptor Interaction Pathway |
| Elastic Fibre Formation Pathway | Elastic Fibre Formation Pathway |
| Endochondral Ossification Pathway | Endochondral Ossification Pathway |
| Extracellular Matrix Organization Pathway | Extracellular Matrix Organization Pathway |
| Hippo Signaling Pathway | Hippo Signaling Pathway |
Diseases associated with Bmp7 ELISA Kit
| Fibrosis | publications with Bmp7 and Fibrosis |
| Kidney Diseases | publications with Bmp7 and Kidney Diseases |
| Inflammation | publications with Bmp7 and Inflammation |
| Cardiovascular Diseases | publications with Bmp7 and Cardiovascular Diseases |
| Carcinoma | publications with Bmp7 and Carcinoma |
| Necrosis | publications with Bmp7 and Necrosis |
| Renal Insufficiency | publications with Bmp7 and Renal Insufficiency |
| Bone Resorption | publications with Bmp7 and Bone Resorption |
| Hypertension | publications with Bmp7 and Hypertension |
| Lung Diseases | publications with Bmp7 and Lung Diseases |
Organs/Tissues associated with Bmp7 ELISA Kit
| Embryonic Tissue | publications with Bmp7 and Embryonic Tissue |
| Brain | publications with Bmp7 and Brain |
| Bone Marrow | publications with Bmp7 and Bone Marrow |
| Eye | publications with Bmp7 and Eye |
| Lung | publications with Bmp7 and Lung |
| Spinal Cord | publications with Bmp7 and Spinal Cord |
| Stomach | publications with Bmp7 and Stomach |
| Pancreas | publications with Bmp7 and Pancreas |
| Adrenal Gland | publications with Bmp7 and Adrenal Gland |
| Dorsal Root Ganglion | publications with Bmp7 and Dorsal Root Ganglion |
