KEY FEATURES
| Full Name | Cluster Of Differentiation 26 |
|---|---|
| Synonym | CD26; ADA; DPPIV; DPP-IV; DPP4; ADABP; ADCP2; TP103; Adenosine Deaminase Complexing Protein 2; T-cell activation antigen CD26; Dipeptidyl Peptidase IV |
| Assay Type | Sandwich |
| Reactivity | Mouse |
| Range | 62.5-4000pg/mL |
| Sensitivity | 27.7pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse CD26 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CD26. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CD26 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CD26, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CD26. You can calculate the concentration of Mouse CD26 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse CD26 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 192.93 | 604.88 | 1680.69 | 202.06 | 589.97 | 1643.56 |
| Standard deviation | 10.84 | 25.04 | 69.75 | 11.92 | 26.08 | 88.59 |
| CV(%) | 5.62 | 4.14 | 4.15 | 5.9 | 4.42 | 5.39 |
RECOVERY
The recovery of Mouse CD26 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 86-97 | 92 |
| EDTA plasma (n=8) | 85-101 | 92 |
| Cell culture media (n=8) | 87-102 | 94 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse CD26 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
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| Ada ELISA Kit | publications with DPP4 and Ada |
| Cxcl12 ELISA Kit | publications with DPP4 and Cxcl12 |
| Tnf ELISA Kit | publications with DPP4 and Tnf |
| Pyy ELISA Kit | publications with DPP4 and Pyy |
| Glp1r ELISA Kit | publications with DPP4 and Glp1r |
| Cxcr3 ELISA Kit | publications with DPP4 and Cxcr3 |
| Cd86 ELISA Kit | publications with DPP4 and Cd86 |
Pathways associated with DPP4 ELISA Kit
| Incretin Synthesis, Secretion, And Inactivation Pathway | Incretin Synthesis, Secretion, And Inactivation Pathway |
| Metabolism Of Proteins Pathway | Metabolism Of Proteins Pathway |
| Peptide Hormone Metabolism Pathway | Peptide Hormone Metabolism Pathway |
| Protein Digestion And Absorption Pathway | Protein Digestion And Absorption Pathway |
| Synthesis, Secretion, And Inactivation Of Glucagon-like Peptide-1 (GLP-1) Pathway | Synthesis, Secretion, And Inactivation Of Glucagon-like Peptide-1 (GLP-1) Pathway |
| Synthesis, Secretion, And Inactivation Of Glucose-dependent Insulinotropic Polypeptide (GIP) Pathway | Synthesis, Secretion, And Inactivation Of Glucose-dependent Insulinotropic Polypeptide (GIP) Pathway |
Diseases associated with DPP4 ELISA Kit
| Cardiovascular Diseases | publications with DPP4 and Cardiovascular Diseases |
| Inflammation | publications with DPP4 and Inflammation |
| Disease Models, Animal | publications with DPP4 and Disease Models, Animal |
| Hyperglycemia | publications with DPP4 and Hyperglycemia |
| Weight Loss | publications with DPP4 and Weight Loss |
| Liver Diseases | publications with DPP4 and Liver Diseases |
| Heart Diseases | publications with DPP4 and Heart Diseases |
| Kidney Diseases | publications with DPP4 and Kidney Diseases |
| Hypertension | publications with DPP4 and Hypertension |
| Adenocarcinoma | publications with DPP4 and Adenocarcinoma |
Organs/Tissues associated with DPP4 ELISA Kit
| Other | publications with DPP4 and Other |
| Pancreas | publications with DPP4 and Pancreas |
| Liver | publications with DPP4 and Liver |
| Heart | publications with DPP4 and Heart |
| Kidney | publications with DPP4 and Kidney |
| Intestine | publications with DPP4 and Intestine |
| Lung | publications with DPP4 and Lung |
| Brain | publications with DPP4 and Brain |
| Skin | publications with DPP4 and Skin |
| Bone Marrow | publications with DPP4 and Bone Marrow |
