KEY FEATURES
| Full Name | Inhibin Beta C |
|---|---|
| Synonym | IHBC; INH-Bc |
| Assay Type | Sandwich |
| Reactivity | Mouse |
| Range | 31.2-2000pg/mL |
| Sensitivity | 12.3pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse INHbC in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse INHbC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse INHbC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse INHbC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse INHbC. You can calculate the concentration of Mouse INHbC in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse INHbC ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 92.01 | 233.82 | 828.63 | 96.85 | 234.84 | 785.92 |
| Standard deviation | 5.57 | 10.57 | 41.6 | 5.49 | 13.76 | 39.85 |
| CV(%) | 6.05 | 4.52 | 5.02 | 5.67 | 5.86 | 5.07 |
RECOVERY
The recovery of Mouse INHbC spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-102 | 96 |
| EDTA plasma (n=8) | 93-107 | 99 |
| Cell culture media (n=8) | 91-102 | 96 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse INHbC in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with INHbC ELISA Kits
| INHBE ELISA Kit | publications with INHbC and INHBE |
| INHBB ELISA Kit | publications with INHbC and INHBB |
| INHA ELISA Kit | publications with INHbC and INHA |
| ACVR1B ELISA Kit | publications with INHbC and ACVR1B |
| INHBA ELISA Kit | publications with INHbC and INHBA |
Pathways associated with INHbC ELISA Kit
| Activin Signaling Pathway | Activin Signaling Pathway |
| Cytokine-cytokine Receptor Interaction Pathway | Cytokine-cytokine Receptor Interaction Pathway |
| Glycoprotein Hormones Pathway | Glycoprotein Hormones Pathway |
| Metabolism Of Proteins Pathway | Metabolism Of Proteins Pathway |
| Peptide Hormone Biosynthesis Pathway | Peptide Hormone Biosynthesis Pathway |
| Peptide Hormone Metabolism Pathway | Peptide Hormone Metabolism Pathway |
| Signaling Pathways Regulating Pluripotency Of Stem Cells | Signaling Pathways Regulating Pluripotency Of Stem Cells |
Diseases associated with INHbC ELISA Kit
| Carcinoma | publications with INHbC and Carcinoma |
| Liver Diseases | publications with INHbC and Liver Diseases |
| Carcinoma, Squamous Cell | publications with INHbC and Carcinoma, Squamous Cell |
| Kidney Diseases | publications with INHbC and Kidney Diseases |
| Liver Cirrhosis, Experimental | publications with INHbC and Liver Cirrhosis, Experimental |
| Hypertrophy | publications with INHbC and Hypertrophy |
| Fibrosis | publications with INHbC and Fibrosis |
| Liver Cirrhosis | publications with INHbC and Liver Cirrhosis |
| Lung Neoplasms | publications with INHbC and Lung Neoplasms |
| Liver Neoplasms | publications with INHbC and Liver Neoplasms |
Organs/Tissues associated with INHbC ELISA Kit
| Liver | publications with INHbC and Liver |
| Ovary | publications with INHbC and Ovary |
| Testis | publications with INHbC and Testis |
