KEY FEATURES
| Full Name | L1-Cell Adhesion Molecule |
|---|---|
| Synonym | CD171; L1-CAM; CAML1; HSAS; HSAS1; MASA; MIC5; N-CAML1; S10; SPG1; Antigen Identified By Monoclonal Antibody R1 |
| Assay Type | Sandwich |
| Reactivity | Mouse |
| Range | 15.6-1000pg/mL |
| Sensitivity | 5.5pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse L1CAM in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse L1CAM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse L1CAM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse L1CAM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse L1CAM. You can calculate the concentration of Mouse L1CAM in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse L1CAM ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 49.58 | 129.79 | 457.15 | 48.9 | 137.82 | 472.91 |
| Standard deviation | 2.64 | 7.4 | 18.24 | 2.9 | 7.66 | 20.24 |
| CV(%) | 5.32 | 5.7 | 3.99 | 5.93 | 5.56 | 4.28 |
RECOVERY
The recovery of Mouse L1CAM spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 93-108 | 99 |
| EDTA plasma (n=8) | 84-99 | 91 |
| Cell culture media (n=8) | 84-96 | 91 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse L1CAM in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Pathways associated with ELISA Kit
| Axon Guidance Pathway | Axon Guidance Pathway |
| CHL1 Interactions Pathway | CHL1 Interactions Pathway |
| Developmental Biology Pathway | Developmental Biology Pathway |
| L1CAM Interactions Pathway | L1CAM Interactions Pathway |
| Splicing Factor NOVA Regulated Synaptic Proteins Pathway | Splicing Factor NOVA Regulated Synaptic Proteins Pathway |
Diseases associated with Chl1 ELISA Kit
| Nervous System Diseases | publications with Chl1 and Nervous System Diseases |
| Mental Disorders | publications with Chl1 and Mental Disorders |
| Schizophrenia | publications with Chl1 and Schizophrenia |
| Neurobehavioral Manifestations | publications with Chl1 and Neurobehavioral Manifestations |
| Intellectual Disability | publications with Chl1 and Intellectual Disability |
| Learning Disorders | publications with Chl1 and Learning Disorders |
| Cardiovascular Diseases | publications with Chl1 and Cardiovascular Diseases |
| Pain | publications with Chl1 and Pain |
| Depressive Disorder, Major | publications with Chl1 and Depressive Disorder, Major |
| Mood Disorders | publications with Chl1 and Mood Disorders |
Organs/Tissues associated with Chl1 ELISA Kit
| Brain | publications with Chl1 and Brain |
| Spinal Cord | publications with Chl1 and Spinal Cord |
| Skin | publications with Chl1 and Skin |
| Dorsal Root Ganglion | publications with Chl1 and Dorsal Root Ganglion |
| Eye | publications with Chl1 and Eye |
| Embryonic Tissue | publications with Chl1 and Embryonic Tissue |
