KEY FEATURES
| Full Name | Myeloperoxidase |
|---|---|
| Synonym | MPO |
| Assay Type | Sandwich |
| Reactivity | Mouse |
| Range | 78-5000pg/mL |
| Sensitivity | 31pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse MPO in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MPO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MPO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MPO. You can calculate the concentration of Mouse MPO in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse MPO ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 230.02 | 498.57 | 1918.54 | 220.91 | 485.86 | 1887.24 |
| Standard deviation | 15.2 | 23.73 | 65.61 | 12.17 | 21.57 | 102.1 |
| CV(%) | 6.61 | 4.76 | 3.42 | 5.51 | 4.44 | 5.41 |
RECOVERY
The recovery of Mouse MPO spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-106 | 100 |
| EDTA plasma (n=8) | 84-99 | 91 |
| Cell culture media (n=8) | 95-107 | 102 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse MPO in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with MPO ELISA Kits
| Tnf ELISA Kit | publications with MPO and Tnf |
| Prtn3 ELISA Kit | publications with MPO and Prtn3 |
| Sod2 ELISA Kit | publications with MPO and Sod2 |
| Il6 ELISA Kit | publications with MPO and Il6 |
| Ctsg ELISA Kit | publications with MPO and Ctsg |
| Apoa1 ELISA Kit | publications with MPO and Apoa1 |
| Il3 ELISA Kit | publications with MPO and Il3 |
| Ltf ELISA Kit | publications with MPO and Ltf |
| Elane ELISA Kit | publications with MPO and Elane |
Pathways associated with MPO ELISA Kit
| Phagosome Pathway | Phagosome Pathway |
| Transcriptional Misregulation In Cancer Pathway | Transcriptional Misregulation In Cancer Pathway |
| Phosphate Acquisition II Pathway | Phosphate Acquisition II Pathway |
Diseases associated with MPO ELISA Kit
| Inflammation | publications with MPO and Inflammation |
| Cardiovascular Diseases | publications with MPO and Cardiovascular Diseases |
| Necrosis | publications with MPO and Necrosis |
| Lung Diseases | publications with MPO and Lung Diseases |
| Reperfusion Injury | publications with MPO and Reperfusion Injury |
| Colitis | publications with MPO and Colitis |
| Kidney Diseases | publications with MPO and Kidney Diseases |
| Edema | publications with MPO and Edema |
| Heart Diseases | publications with MPO and Heart Diseases |
| Hemorrhage | publications with MPO and Hemorrhage |
Organs/Tissues associated with MPO ELISA Kit
| Blood | publications with MPO and Blood |
| Lung | publications with MPO and Lung |
| Kidney | publications with MPO and Kidney |
| Brain | publications with MPO and Brain |
| Bone | publications with MPO and Bone |
| Bone Marrow | publications with MPO and Bone Marrow |
| Eye | publications with MPO and Eye |
| Lymph Node | publications with MPO and Lymph Node |
| Thyroid | publications with MPO and Thyroid |
| Embryonic Tissue | publications with MPO and Embryonic Tissue |
