KEY FEATURES
| Full Name | Neutrophil Elastase |
|---|---|
| Synonym | ELANE; HLE; HNE; ELA2; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Human Leukocyte Elastase; PMN Elastase |
| Assay Type | Sandwich |
| Reactivity | Mouse |
| Range | 0.15-10ng/mL |
| Sensitivity | 0.07ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse NE in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NE. You can calculate the concentration of Mouse NE in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse NE ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.46 | 0.96 | 4.01 | 0.42 | 0.96 | 3.7 |
| Standard deviation | 0.02 | 0.05 | 0.19 | 0.03 | 0.05 | 0.18 |
| CV(%) | 5.34 | 5.64 | 4.73 | 6.49 | 5.27 | 4.9 |
RECOVERY
The recovery of Mouse NE spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 83-97 | 90 |
| EDTA plasma (n=8) | 87-98 | 93 |
| Cell culture media (n=8) | 87-101 | 93 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse NE in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with NE ELISA Kits
| SLC18A2 ELISA Kit | publications with NE and SLC18A2 |
| PICK1 ELISA Kit | publications with NE and PICK1 |
| STX1A ELISA Kit | publications with NE and STX1A |
Pathways associated with NE ELISA Kit
| Monoamine Transport Pathway | Monoamine Transport Pathway |
| Na+/Cl- Dependent Neurotransmitter Transporters Pathway | Na+/Cl- Dependent Neurotransmitter Transporters Pathway |
| SLC-mediated Transmembrane Transport Pathway | SLC-mediated Transmembrane Transport Pathway |
| Transmembrane Transport Of Small Molecules Pathway | Transmembrane Transport Of Small Molecules Pathway |
| Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway | Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway |
Diseases associated with NE ELISA Kit
| Mental Disorders | publications with NE and Mental Disorders |
| Nervous System Diseases | publications with NE and Nervous System Diseases |
| Attention Deficit Disorder with Hyperactivity | publications with NE and Attention Deficit Disorder with Hyperactivity |
| Cardiovascular Diseases | publications with NE and Cardiovascular Diseases |
| Depressive Disorder | publications with NE and Depressive Disorder |
| Heart Diseases | publications with NE and Heart Diseases |
| Brain Diseases | publications with NE and Brain Diseases |
| Hypertension | publications with NE and Hypertension |
| Disease Models, Animal | publications with NE and Disease Models, Animal |
| Tachycardia | publications with NE and Tachycardia |
Organs/Tissues associated with NE ELISA Kit
| Brain | publications with NE and Brain |
| Nerve | publications with NE and Nerve |
| Muscle | publications with NE and Muscle |
| Kidney | publications with NE and Kidney |
| Placenta | publications with NE and Placenta |
| Intestine | publications with NE and Intestine |
| Testis | publications with NE and Testis |
| Prostate | publications with NE and Prostate |
