KEY FEATURES
| Full Name | Plasminogen Activator Inhibitor 1 |
|---|---|
| Synonym | SERPINE1; PLANH1; Serpin Peptidase Inhibitor Clade E Member 1; Nexin,Plasminogen Activator Inhibitor Type 1 Serpin E1; Endothelial plasminogen activator inhibitor |
| Assay Type | Sandwich |
| Reactivity | Mouse |
| Range | 31.2-2000pg/mL |
| Sensitivity | 12.3pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse PAI1 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PAI1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PAI1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PAI1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PAI1. You can calculate the concentration of Mouse PAI1 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse PAI1 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 104.46 | 258.39 | 985.15 | 100.81 | 238.02 | 954.47 |
| Standard deviation | 6.72 | 14.88 | 43.35 | 5.88 | 10.57 | 40.95 |
| CV(%) | 6.43 | 5.76 | 4.4 | 5.83 | 4.44 | 4.29 |
RECOVERY
The recovery of Mouse PAI1 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 86-99 | 92 |
| EDTA plasma (n=8) | 89-102 | 94 |
| Cell culture media (n=8) | 95-110 | 101 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse PAI1 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with PAI ELISA Kits
| TGFB1 ELISA Kit | publications with PAI and TGFB1 |
| SMAD4 ELISA Kit | publications with PAI and SMAD4 |
| HIF1A ELISA Kit | publications with PAI and HIF1A |
| EGF ELISA Kit | publications with PAI and EGF |
| PLG ELISA Kit | publications with PAI and PLG |
| PLAT ELISA Kit | publications with PAI and PLAT |
| PLAU ELISA Kit | publications with PAI and PLAU |
| VTN ELISA Kit | publications with PAI and VTN |
| ALB ELISA Kit | publications with PAI and ALB |
Pathways associated with PAI ELISA Kit
| Adipogenesis Pathway | Adipogenesis Pathway |
| BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway | BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway |
| Blood Clotting Cascade Pathway | Blood Clotting Cascade Pathway |
| Chagas Disease (American Trypanosomiasis) Pathway | Chagas Disease (American Trypanosomiasis) Pathway |
| Circadian Clock Pathway | Circadian Clock Pathway |
| Complement And Coagulation Cascades Pathway | Complement And Coagulation Cascades Pathway |
| Direct P53 Effectors Pathway | Direct P53 Effectors Pathway |
Diseases associated with PAI ELISA Kit
| Cardiovascular Diseases | publications with PAI and Cardiovascular Diseases |
| Thrombosis | publications with PAI and Thrombosis |
| Inflammation | publications with PAI and Inflammation |
| Heart Diseases | publications with PAI and Heart Diseases |
| Necrosis | publications with PAI and Necrosis |
| Atherosclerosis | publications with PAI and Atherosclerosis |
| Hypertension | publications with PAI and Hypertension |
| Fibrosis | publications with PAI and Fibrosis |
| Kidney Diseases | publications with PAI and Kidney Diseases |
| Nervous System Diseases | publications with PAI and Nervous System Diseases |
Organs/Tissues associated with PAI ELISA Kit
| Blood | publications with PAI and Blood |
| Vascular | publications with PAI and Vascular |
| Heart | publications with PAI and Heart |
| Kidney | publications with PAI and Kidney |
| Muscle | publications with PAI and Muscle |
| Liver | publications with PAI and Liver |
| Connective Tissue | publications with PAI and Connective Tissue |
| Lung | publications with PAI and Lung |
| Adipose Tissue | publications with PAI and Adipose Tissue |
| Brain | publications with PAI and Brain |
