KEY FEATURES
| Full Name | Programmed Cell Death Protein 1 Ligand 1 |
|---|---|
| Synonym | CD274; PDCD1LG1; B7-H; B7H1; PD-L1; PDCD1L1; PDCD1-LG1; Programed Death Ligand 1 |
| Assay Type | Sandwich |
| Reactivity | Mouse |
| Range | 0.15-10ng/mL |
| Sensitivity | 0.06ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse PDL1 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PDL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PDL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PDL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PDL1. You can calculate the concentration of Mouse PDL1 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse PDL1 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.49 | 0.84 | 4.07 | 0.52 | 0.84 | 3.69 |
| Standard deviation | 0.03 | 0.05 | 0.14 | 0.03 | 0.05 | 0.15 |
| CV(%) | 6.72 | 5.93 | 3.52 | 6.25 | 5.57 | 4.01 |
RECOVERY
The recovery of Mouse PDL1 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 92-105 | 99 |
| EDTA plasma (n=8) | 89-101 | 96 |
| Cell culture media (n=8) | 95-110 | 101 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse PDL1 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with Cd274 ELISA Kits
| Pdcd1 ELISA Kit | publications with Cd274 and Pdcd1 |
| Cd80 ELISA Kit | publications with Cd274 and Cd80 |
| Cd4 ELISA Kit | publications with Cd274 and Cd4 |
| Pdcd1lg2 ELISA Kit | publications with Cd274 and Pdcd1lg2 |
| Stat1 ELISA Kit | publications with Cd274 and Stat1 |
| Ptpn6 ELISA Kit | publications with Cd274 and Ptpn6 |
| Ptpn11 ELISA Kit | publications with Cd274 and Ptpn11 |
Pathways associated with Cd274 ELISA Kit
| Adaptive Immune System Pathway | Adaptive Immune System Pathway |
| Cell Adhesion Molecules (CAMs) Pathway | Cell Adhesion Molecules (CAMs) Pathway |
| Costimulation By The CD28 Family Pathway | Costimulation By The CD28 Family Pathway |
| Immune System Pathway | Immune System Pathway |
| PD-1 Signaling Pathway | PD-1 Signaling Pathway |
Diseases associated with Cd274 ELISA Kit
| Disease Models, Animal | publications with Cd274 and Disease Models, Animal |
| Inflammation | publications with Cd274 and Inflammation |
| Lung Neoplasms | publications with Cd274 and Lung Neoplasms |
| Lung Diseases | publications with Cd274 and Lung Diseases |
| Adenocarcinoma | publications with Cd274 and Adenocarcinoma |
| Liver Diseases | publications with Cd274 and Liver Diseases |
| Necrosis | publications with Cd274 and Necrosis |
| Neoplasms, Experimental | publications with Cd274 and Neoplasms, Experimental |
| Nervous System Diseases | publications with Cd274 and Nervous System Diseases |
| Kidney Diseases | publications with Cd274 and Kidney Diseases |
Organs/Tissues associated with Cd274 ELISA Kit
| Blood | publications with Cd274 and Blood |
| Lung | publications with Cd274 and Lung |
| Bone Marrow | publications with Cd274 and Bone Marrow |
| Spleen | publications with Cd274 and Spleen |
| Skin | publications with Cd274 and Skin |
| Pancreas | publications with Cd274 and Pancreas |
| Heart | publications with Cd274 and Heart |
| Stomach | publications with Cd274 and Stomach |
| Uterus | publications with Cd274 and Uterus |
| Thymus | publications with Cd274 and Thymus |
