KEY FEATURES
| Full Name | Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide |
|---|---|
| Synonym | PI3K2a; CPK; PI3-K-C2(Alpha); PI3-K-C2A; PI3K2-a; Phosphoinositide 3-Kinase 2a; Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha |
| Assay Type | Sandwich |
| Reactivity | Mouse |
| Range | 0.15-10ng/mL |
| Sensitivity | 0.06ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse PIK3C2a in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PIK3C2a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PIK3C2a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PIK3C2a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PIK3C2a. You can calculate the concentration of Mouse PIK3C2a in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse PIK3C2a ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.5 | 1.45 | 4.76 | 0.45 | 1.37 | 5.22 |
| Standard deviation | 0.03 | 0.08 | 0.14 | 0.03 | 0.07 | 0.25 |
| CV(%) | 5.29 | 5.4 | 3.04 | 6.71 | 4.82 | 4.78 |
RECOVERY
The recovery of Mouse PIK3C2a spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-108 | 100 |
| EDTA plasma (n=8) | 95-110 | 101 |
| Cell culture media (n=8) | 88-104 | 96 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse PIK3C2a in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Pathways associated with ELISA Kit
| Clathrin Derived Vesicle Budding Pathway | Clathrin Derived Vesicle Budding Pathway |
| Golgi Associated Vesicle Biogenesis Pathway | Golgi Associated Vesicle Biogenesis Pathway |
| Inositol Phosphate Metabolism Pathway | Inositol Phosphate Metabolism Pathway |
| Insulin Signaling Pathway | Insulin Signaling Pathway |
| Membrane Trafficking Pathway | Membrane Trafficking Pathway |
| Phosphatidylinositol Signaling System Pathway | Phosphatidylinositol Signaling System Pathway |
| Regulation Of Actin Cytoskeleton Pathway | Regulation Of Actin Cytoskeleton Pathway |
| Trans-Golgi Network Vesicle Budding Pathway | Trans-Golgi Network Vesicle Budding Pathway |
Diseases associated with PIK3C2A ELISA Kit
| Depressive Disorder | publications with PIK3C2A and Depressive Disorder |
| Renal Insufficiency | publications with PIK3C2A and Renal Insufficiency |
| Neoplasm Metastasis | publications with PIK3C2A and Neoplasm Metastasis |
| Breast Neoplasms | publications with PIK3C2A and Breast Neoplasms |
| Prostatic Neoplasms | publications with PIK3C2A and Prostatic Neoplasms |
| Kidney Diseases | publications with PIK3C2A and Kidney Diseases |
| Prostatic Diseases | publications with PIK3C2A and Prostatic Diseases |
Organs/Tissues associated with PIK3C2A ELISA Kit
| Brain | publications with PIK3C2A and Brain |
| Prostate | publications with PIK3C2A and Prostate |
| Bone | publications with PIK3C2A and Bone |
| Bone Marrow | publications with PIK3C2A and Bone Marrow |
| Ovary | publications with PIK3C2A and Ovary |
| Kidney | publications with PIK3C2A and Kidney |
| Pancreas | publications with PIK3C2A and Pancreas |
