KEY FEATURES
| Full Name | Tumor Necrosis Factor Receptor Superfamily, Member 17 |
|---|---|
| Synonym | CD269; BCM; BCMA; B-cell maturation protein |
| Assay Type | Sandwich |
| Reactivity | Mouse |
| Range | 0.31-20ng/mL |
| Sensitivity | 0.11ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Mouse TNFRSF17 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNFRSF17. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNFRSF17 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNFRSF17, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TNFRSF17. You can calculate the concentration of Mouse TNFRSF17 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Mouse TNFRSF17 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.94 | 2.09 | 9 | 0.85 | 2.14 | 8.65 |
| Standard deviation | 0.06 | 0.11 | 0.46 | 0.05 | 0.09 | 0.38 |
| CV(%) | 6.27 | 5.28 | 5.15 | 5.37 | 4.14 | 4.44 |
RECOVERY
The recovery of Mouse TNFRSF17 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 91-108 | 98 |
| EDTA plasma (n=8) | 93-106 | 100 |
| Cell culture media (n=8) | 91-101 | 96 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Mouse TNFRSF17 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with BCMA ELISA Kits
| Tnfsf13b antibody | publications with BCMA and Tnfsf13b |
| Tnfrsf13b antibody | publications with BCMA and Tnfrsf13b |
| Tnfrsf13c antibody | publications with BCMA and Tnfrsf13c |
| Tnfsf12 antibody | publications with BCMA and Tnfsf12 |
| Traf3 antibody | publications with BCMA and Traf3 |
| Mzb1 antibody | publications with BCMA and Mzb1 |
| Dleu7 antibody | publications with BCMA and Dleu7 |
Pathways associated with BCMA ELISA Kit
| Cytokine Signaling In Immune System Pathway | Cytokine Signaling In Immune System Pathway |
| Cytokine-cytokine Receptor Interaction Pathway | Cytokine-cytokine Receptor Interaction Pathway |
| Immune System Pathway | Immune System Pathway |
| Intestinal Immune Network For IgA Production Pathway | Intestinal Immune Network For IgA Production Pathway |
| TNFR2 Non-canonical NF-kB Pathway | TNFR2 Non-canonical NF-kB Pathway |
| TNFs Bind Their Physiological Receptors Pathway | TNFs Bind Their Physiological Receptors Pathway |
Diseases associated with BCMA ELISA Kit
| Leukemia | publications with BCMA and Leukemia |
| Necrosis | publications with BCMA and Necrosis |
| Inflammation | publications with BCMA and Inflammation |
| Death | publications with BCMA and Death |
| Cardiovascular Diseases | publications with BCMA and Cardiovascular Diseases |
| Multiple Myeloma | publications with BCMA and Multiple Myeloma |
| Cell Transformation, Neoplastic | publications with BCMA and Cell Transformation, Neoplastic |
| Nervous System Diseases | publications with BCMA and Nervous System Diseases |
| Skin Diseases | publications with BCMA and Skin Diseases |
| Neoplasms, Experimental | publications with BCMA and Neoplasms, Experimental |
Organs/Tissues associated with BCMA ELISA Kit
| Intestine | publications with BCMA and Intestine |
| Stomach | publications with BCMA and Stomach |
| Mammary Gland | publications with BCMA and Mammary Gland |
