Product description
This ELISA kit is rapid, quantitative enzyme-linked immunosorbent assays (ELISA) for the determination of Phenylethanolamine A content in Food samples.
Key features
Assay Range: 0.1 ppb~2.4 ppb
Sensitivity: 0.1 ppb (ng/mL)
Species reactivity: Universal
Assay time: 45min
Result type: Quantitative
Category: Residues&contaminants
Kit type: ELISA
Detection type: Competitive
Precision: CV<10%
Recovery rate: 100%±30%
Specificity:
Phenylethanolamine A | 100.0% | ||||
Clenpenterol, Dobutamine, Zilpaterol, Cimbuterol, Mabuterol, Terbutaline, Penbutolol, Brombuterol, Tulobuterol, Clorprenaline, Cimaterol, Clenbuterol, Ractopamine, Salbutamol | ＜0.1% |
Detection limits:
Pig urine | 0.5 ppb |
Storage and shelf life
The kit should be stored at 2~8°C and must not be frozen. The shelf life of this product is 1 year. Do not use the kit beyond the expiration date.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The kit consists of an enzyme-labeled plate pre-coated with coupled antigens, horseradish enzyme markers, antibodies, standard substances, and other supporting reagents. During detection, standard substances or sample solutions are added. Phenylethanolamine A in the samples competes with the pre-coated coupled antigens on the enzyme-labeled plate for Phenylethanolamine A specific antibody. After adding the enzyme markers, TMB substrate is used for color development. The absorbance value of the sample is negatively correlated with the content of Phenylethanolamine A it contains, and the residual amount of Phenylethanolamine A in the sample can be obtained by comparing with the standard curve.
Materials supplied
Item | Quantity | ||||
Assay Plate | 96T | ||||
Standard | 6 x 1.5 mL | ||||
High concentration standard (100 ppb) | 1 x 1 mL | ||||
HRP Conjugate | 1 x 7 mL | ||||
Antibody | 1 x 7 mL | ||||
TMB Substrate Reagent (A\B) | 2 x 7 mL | ||||
Stop Solution | 1 x 7 mL | ||||
Wash Buffer (20×) | 1 x 25 mL | ||||
Plate Sealer | 1 piece | ||||
Instruction Manual | 1 piece | ||||
Sealed Bag | 1 copy |
Standard curve reference
Standard | 1 | 2 | 3 | 4 | 5 | 6 | |||
Concentration (ppb=ng/mL=ng/g) | 0 | 0.1 | 0.3 | 0.6 | 1.2 | 2.4 | |||
OD Standard | 2.074 | 1.587 | 0.885 | 0.370 | 0.149 | 0.072 |
