KEY FEATURES
| Full Name | Growth Regulated Oncogene Alpha |
|---|---|
| Synonym | GROα/CXCL1 |
| Assay Type | Sandwich |
| Reactivity | Porcine |
| Range | 15.63-1000pg/mL |
| Sensitivity | 9.38pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Porcine GROα/CXCL1 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine GROα/CXCL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine GROα/CXCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine GROα/CXCL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine GROα/CXCL1. You can calculate the concentration of Porcine GROα/CXCL1 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Porcine GROα/CXCL1 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 48.86 | 146.81 | 345.23 | 43.99 | 139.67 | 363.49 |
| Standard deviation | 3.04 | 6.5 | 18.88 | 2.61 | 8.09 | 14.9 |
| CV(%) | 6.22 | 4.43 | 5.47 | 5.94 | 5.79 | 4.1 |
RECOVERY
The recovery of Porcine GROα/CXCL1 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 96-109 | 101 |
| EDTA plasma (n=8) | 85-97 | 91 |
| Cell culture media (n=8) | 91-104 | 96 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Porcine GROα/CXCL1 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with GROA ELISA Kits
| CXCL2 ELISA Kit | publications with GROA and CXCL2 |
| CXCR2 ELISA Kit | publications with GROA and CXCR2 |
| CCL5 ELISA Kit | publications with GROA and CCL5 |
| CXCL3 ELISA Kit | publications with GROA and CXCL3 |
| CXCR1 ELISA Kit | publications with GROA and CXCR1 |
| CCL20 ELISA Kit | publications with GROA and CCL20 |
| CXCL6 ELISA Kit | publications with GROA and CXCL6 |
| IL6 ELISA Kit | publications with GROA and IL6 |
| CXCL11 ELISA Kit | publications with GROA and CXCL11 |
| DARC ELISA Kit | publications with GROA and DARC |
Pathways associated with GROA ELISA Kit
| Amoebiasis Pathway | Amoebiasis Pathway |
| Chemokine Receptors Bind Chemokines Pathway | Chemokine Receptors Bind Chemokines Pathway |
| Chemokine Signaling Pathway | Chemokine Signaling Pathway |
| Class A/1 (Rhodopsin-like Receptors) Pathway | Class A/1 (Rhodopsin-like Receptors) Pathway |
| Cytokine-cytokine Receptor Interaction Pathway | Cytokine-cytokine Receptor Interaction Pathway |
| Cytokines And Inflammatory Response Pathway | Cytokines And Inflammatory Response Pathway |
| Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway | Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway |
Diseases associated with GROA ELISA Kit
| Inflammation | publications with GROA and Inflammation |
| Necrosis | publications with GROA and Necrosis |
| Disease Models, Animal | publications with GROA and Disease Models, Animal |
| Lung Diseases | publications with GROA and Lung Diseases |
| Cardiovascular Diseases | publications with GROA and Cardiovascular Diseases |
| Lung Injury | publications with GROA and Lung Injury |
| Pneumonia | publications with GROA and Pneumonia |
| Reperfusion Injury | publications with GROA and Reperfusion Injury |
| Wounds and Injuries | publications with GROA and Wounds and Injuries |
| Acute Lung Injury | publications with GROA and Acute Lung Injury |
Organs/Tissues associated with GROA ELISA Kit
| Blood | publications with GROA and Blood |
| Lung | publications with GROA and Lung |
| Vascular | publications with GROA and Vascular |
| Brain | publications with GROA and Brain |
| Liver | publications with GROA and Liver |
| Skin | publications with GROA and Skin |
| Intestine | publications with GROA and Intestine |
| Kidney | publications with GROA and Kidney |
| Heart | publications with GROA and Heart |
| Eye | publications with GROA and Eye |
