KEY FEATURES
| Full Name | Procollagen I C-terminal Propeptide |
|---|---|
| Synonym | PICP |
| Assay Type | Sandwich |
| Reactivity | Porcine |
| Range | 62.5-4000pg/mL |
| Sensitivity | 37.5pg/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Porcine PICP in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine PICP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine PICP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine PICP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine PICP. You can calculate the concentration of Porcine PICP in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Porcine PICP ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 211.05 | 608.28 | 1642.63 | 223.82 | 595.19 | 1771.78 |
| Standard deviation | 13.68 | 29.2 | 65.87 | 15.38 | 34.4 | 85.93 |
| CV(%) | 6.48 | 4.8 | 4.01 | 6.87 | 5.78 | 4.85 |
RECOVERY
The recovery of Porcine PICP spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 86-98 | 92 |
| EDTA plasma (n=8) | 95-110 | 101 |
| Cell culture media (n=8) | 87-99 | 93 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Porcine PICP in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with PICP ELISA Kits
| BMP1 ELISA Kit | publications with PICP and BMP1 |
| COL1A2 ELISA Kit | publications with PICP and COL1A2 |
| ADAMTS14 ELISA Kit | publications with PICP and ADAMTS14 |
| ADAMTS2 ELISA Kit | publications with PICP and ADAMTS2 |
Pathways associated with PICP ELISA Kit
| Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway | Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway |
| Collagen Biosynthesis And Modifying Enzymes Pathway | Collagen Biosynthesis And Modifying Enzymes Pathway |
| Collagen Formation Pathway | Collagen Formation Pathway |
| Crosslinking Of Collagen Fibrils Pathway | Crosslinking Of Collagen Fibrils Pathway |
| Extracellular Matrix Organization Pathway | Extracellular Matrix Organization Pathway |
Diseases associated with PICP ELISA Kit
| Fibrosis | publications with PICP and Fibrosis |
| Nervous System Diseases | publications with PICP and Nervous System Diseases |
| Cardiovascular Diseases | publications with PICP and Cardiovascular Diseases |
| Heart Diseases | publications with PICP and Heart Diseases |
| Necrosis | publications with PICP and Necrosis |
| Liver Diseases | publications with PICP and Liver Diseases |
| Skin Diseases | publications with PICP and Skin Diseases |
| Breast Neoplasms | publications with PICP and Breast Neoplasms |
| Inflammation | publications with PICP and Inflammation |
| Hyperplasia | publications with PICP and Hyperplasia |
Organs/Tissues associated with PICP ELISA Kit
| Bone | publications with PICP and Bone |
| Muscle | publications with PICP and Muscle |
| Heart | publications with PICP and Heart |
| Connective Tissue | publications with PICP and Connective Tissue |
| Embryonic Tissue | publications with PICP and Embryonic Tissue |
| Eye | publications with PICP and Eye |
| Liver | publications with PICP and Liver |
| Brain | publications with PICP and Brain |
| Kidney | publications with PICP and Kidney |
| Uterus | publications with PICP and Uterus |
