KEY FEATURES
| Full Name | Insulin Like Growth Factor 1 |
|---|---|
| Synonym | IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor |
| Assay Type | Sandwich |
| Reactivity | Rat |
| Range | 0.15-10ng/mL |
| Sensitivity | 0.07ng/mL |
| Sample Type | Serum,plasma and other biological fluids |
| Sample Volume | 100μL |
| Detection Wavelength | OD450 |
| Transportation Temperature | 2-8°C |
| Specificity | The kit detected Rat IGF1 in the samples and no significant cross-species reactions were found |
| Microplate | 96-wells plate breakable into 12 x 8 wells strip |
TEST PRINCIPLE
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IGF1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IGF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IGF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IGF1. You can calculate the concentration of Rat IGF1 in the samples by comparing the OD of the samples to the standard curve. |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 120μL×1 vial 48T: 60μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 14mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 14mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 10mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
ASSAY PROCEDURES
TYPICAL DATA
Rat IGF1 ELISA Standard Curve
Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.
PRECISION
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.53 | 0.92 | 3.9 | 0.52 | 0.88 | 3.97 |
| Standard deviation | 0.03 | 0.04 | 0.16 | 0.04 | 0.04 | 0.16 |
| CV(%) | 5.78 | 4.23 | 4.05 | 6.86 | 5.09 | 3.92 |
RECOVERY
The recovery of Rat IGF1 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 93-106 | 98 |
| EDTA plasma (n=8) | 85-100 | 92 |
| Cell culture media (n=8) | 88-101 | 95 |
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Rat IGF1 in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
Products associated with IGF ELISA Kits
| IGF1R ELISA Kit | publications with IGF and IGF1R |
| IGFBP3 ELISA Kit | publications with IGF and IGFBP3 |
| IGFBP1 ELISA Kit | publications with IGF and IGFBP1 |
| IRS1 ELISA Kit | publications with IGF and IRS1 |
| IGFBP2 ELISA Kit | publications with IGF and IGFBP2 |
| INSR ELISA Kit | publications with IGF and INSR |
| TGFB1 ELISA Kit | publications with IGF and TGFB1 |
| IGFBP5 ELISA Kit | publications with IGF and IGFBP5 |
| INS ELISA Kit | publications with IGF and INS |
| IGFBP4 ELISA Kit | publications with IGF and IGFBP4 |
Pathways associated with IGF ELISA Kit
| AMPK Signaling Pathway | AMPK Signaling Pathway |
| Adipogenesis Pathway | Adipogenesis Pathway |
| Aldosterone-regulated Sodium Reabsorption Pathway | Aldosterone-regulated Sodium Reabsorption Pathway |
| Apoptosis Pathway | Apoptosis Pathway |
| Cardiac Progenitor Differentiation Pathway | Cardiac Progenitor Differentiation Pathway |
| Dilated Cardiomyopathy Pathway | Dilated Cardiomyopathy Pathway |
| Endochondral Ossification Pathway | Endochondral Ossification Pathway |
Diseases associated with IGF ELISA Kit
| Acromegaly | publications with IGF and Acromegaly |
| Breast Neoplasms | publications with IGF and Breast Neoplasms |
| Inflammation | publications with IGF and Inflammation |
| Female Urogenital Diseases | publications with IGF and Female Urogenital Diseases |
| Adenoma | publications with IGF and Adenoma |
| Prostatic Neoplasms | publications with IGF and Prostatic Neoplasms |
| Necrosis | publications with IGF and Necrosis |
| Pituitary Neoplasms | publications with IGF and Pituitary Neoplasms |
| Hypertension | publications with IGF and Hypertension |
| Kidney Diseases | publications with IGF and Kidney Diseases |
Organs/Tissues associated with IGF ELISA Kit
| Blood | publications with IGF and Blood |
| Muscle | publications with IGF and Muscle |
| Liver | publications with IGF and Liver |
| Bone | publications with IGF and Bone |
| Brain | publications with IGF and Brain |
| Vascular | publications with IGF and Vascular |
| Ovary | publications with IGF and Ovary |
| Heart | publications with IGF and Heart |
| Connective Tissue | publications with IGF and Connective Tissue |
| Lung | publications with IGF and Lung |
