Product description
This ELISA kit is rapid, quantitative enzyme-linked immunosorbent assays (ELISA) for the determination of Sulbactam content in Food samples.
Key features
Sensitivity: 0.5 ppb (ng/mL)
Species reactivity: Universal
Assay time: 75min
Result type: Quantitative
Category: Residues&contaminants
Kit type: ELISA
Detection type: Competitive
Precision: CV<10%
Detection limits:
Raw milk | 50 ppb |
Storage and shelf life
The kit should be stored at 2~8°C and must not be frozen. The shelf life of this product is 1 year. Do not use the kit beyond the expiration date.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The kit consists of an enzyme-labeled plate pre-coated with coupled antigens, β-Lactamase Lyophilized Microplate, reaction solution, and other supporting reagents. During detection, negative control or sample solutions are added to the β-Lactamase Lyophilized Microplate, and reaction solution A is added to complete the specific reaction. Then transfer the reaction solution to the sulbactam microplate and add reaction solution B. The substrate reagent is added to initiate the color developing reaction. The presence of SUL can be determined according to the OD value by using a microplate reader with 450 nm (630 nm) wavelength.
Materials supplied
Item | Quantity | ||||
Sulbactam ELISA Plate | 96T | ||||
β-Lactamase Lyophilized Microplate | 96 wells | ||||
Lyophilized microplate of reaction solution A | 24 wells | ||||
Concentrated reaction solution B (5×) | 1 x 1.5 mL | ||||
Diluted reaction solution B | 1 x 6 mL | ||||
Negative Control | 1 x 5 mL | ||||
TMB Substrate Reagent (A\B) | 2 x 7 mL | ||||
Stop Solution | 1 x 7 mL | ||||
Wash Buffer (20×) | 1 x 25 mL | ||||
Plate Sealer | 1 piece | ||||
Instruction Manual | 1 piece | ||||
Sealed Bag | 1 copy |
