Total protein (TP) Assay Kit (Coomassie Brilliant Blue Method)

Total protein (TP) Assay Kit (Coomassie Brilliant Blue Method)

Cat: BC00007
Size1:100T Price1:$160
Range:0.0625-1mg/ml
Sensitivity:0.0268mg/ml
Detection Method:Colorimetric;Quantitative
Detection Instrument:Microplate reader(560-610 nm,optimum wavelength:595 nm)
Sample Type:serum, plasma, Urine, Animal and Plant Tissues, Culture Medium, cells
Category: Metabolism Kits Tags: , ,

Product Introduction

This kit is a kit commonly used to determine the protein content in biological samples. Coomassie blue staining, also known as Bradford method, is a method for determining protein concentration.


Principle

Coomassie brilliant blue G-250 is red in the free state, with the maximum light absorption at 465nm; when it binds to protein, it turns cyan, and the protein-pigment complex has the maximum light absorption at a wavelength of 595 nm. The depth of its color is proportional to the protein content.

Components

Serial number

Components

Size(100T)

Storage

Reagent 1

Chromogen Stock Solution

15 mL

-20, avoid light, store at 2-8 after opening

Reagent 1

1 mg/mL Standard

1.5 mL

-20, avoid light, store at room temperature after opening

Consumable 1

Microplate(96 wells)

1 plate

RT

Consumable 2

Plate Sealer

2 pieces

RT


Storage

The unopened kit can be stored at -20 for 12 months.

After opening the kit,please store it according to the requirements of "Components-Storage", valid for 6 months.

   💬 WhatsApp