The EnkiLife FITC labeling kit includes multiple components for labeling antibody proteins, such as high - performance fluorescent dye, ultrafiltration centrifuge tubes for purification, and labeling buffers. It enables easy and reliable labeling of antibodies and proteins.
Features
Fast: Labeling takes only about 30 minutes, balancing labeling effectiveness and efficiency.
Convenient: Each dye reagent is pre - optimized for corresponding antibody amounts, eliminating complex calculations. The solid dye form ensures batch stability, and following the steps yields good results.
Flexible: In the large - scale kit, the dye is pre - portioned for consistent batch performance, making it convenient for multiple labeling uses.
Outstanding labeling performance: The optimized labeling buffer ensures relatively fixed dye labeling sites, enhancing the homogeneity of labeled antibodies.
Amine - specific labeling: The activating group on the fluorescent dye efficiently labels antibodies and other purified proteins at primary amines.
Labeled proteins: This kit is mainly designed for IgG antibody labeling. Proteins similar in size and properties to IgG antibodies can also be labeled.
Fluorescent dye Introduction
Classic fluorescent dyes, the most commonly used and have a wide range of applications, including in flow cytometry and immunofluorescence.
Properties of FITC
Similar Dyes: Cy2,Fluorescein-X,FAM,Alexa Fluor488
Excitation Wavelength: 495nm
Emission Wavelength: 520nm
Molar Extinction Coefficient (L/(mol・cm)): 68000
Purity: > 95%
Solubility: Soluble in organic solvents like DMSO and DMF
FITC Spectrum
Kit Specifications
Product composition | Content of components in different specifications | Storage temperature | ||
40 μg antibody | 200 μg antibody | 2 mg antibody | ||
FITC | 1 tube | 5 tubes | 5 tubes | -20 °C after opening, away from light |
Ultrafiltration Tube* ,50K MWCO | 1 set ** | 1 set ** | 1 set ** | RT |
Labeling Buffer S | 10 mL | 10 mL | 10 mL | 2-8 °C |
1× PBS (pH 7.4) | 10 mL | 10 mL | 10 mL | 2-8 °C |
DMF | 100 μL | 100 μL | 100 μL | 2-8 °C, away from light |
Labeled protein storage solution | 200 μL | 1 mL | 5 mL | 2-8 °C |
Recommended amount of labeled antibody | Each tube is labeled with 20-40 μg, and the best is 20 μg | Each tube is labeled with 20-40 μg, and the best is 20 μg | Each tube is labeled with 100-400 μg, and the best is 200 μg | |
Application Examples of FITC
Labeled antibody was used as an immunofluorescent probe
WB
FRAP
FITC - Antibody Labeling Principle
Within a certain pH range, the activated group of the fluorescent dye specifically reacts with primary amino groups on antibody proteins to form stable amide bonds, thereby conjugating the dye to the antibody protein.
Conventional Antibody Protein Labeling Process
Step 1：Ultrafiltration buffer exchange and concentration
Step 2：Labeling reaction
Step 3：Purification
Step 4：DOL calculation and collection preservation
