Necroptosis
Necroptosis, a regulated pathway for necrotic cell death, is triggered by a number of inflammatory signals, including cytokines in the tumor necrosis factor (TNF) family, pathogen sensors such as toll-like receptors (TLRs), ischemic injury, and neurodegenerative diseases. The process is negatively regulated by caspases and is initiated through a complex containing the RIP and RIP3 kinases, typically referred to as the necrosome. Necroptosis is inhibited by a small molecule inhibitor of RIP, necrostatin-1 (Nec-1). RIP is phosphorylated at several sites within the kinase domain that are sensitive to Nec-1, including Ser14, Ser15, Ser161, and Ser166. During necroptosis, RIP3 is phosphorylated at Ser227, leading to recruitment and phosphorylation of MLKL at Thr357 and Ser358. Phosphorylation of MLKL results in its oligomerization and translocation to the plasma membrane, where it affects membrane integrity.
Relevent antibodies
| Catalog# | Product Name | Reactivity | Application |
|---|---|---|---|
| AMRe21588 | RIP Rabbit Monoclonal antibody | Human | WB,IHC,IF,IP,ELISA |
| APRab20453 | RIP (Phospho Ser166) Rabbit Polyclonal Antibody | Human,Rat,Mouse | WB,IHC |
| AMRe21172 | MLKL Rabbit Monoclonal antibody | Human,Mouse,Rat | WB,IHC,IF,IP,ELISA |
| AMRe84013 | Phospho-MLKL(S358) Rabbit Monoclonal | Human | WB,IHC |
| AMM83568 | RIPK3 Mouse Monoclonal Antibody | Human | IHC |
| APS0635 | HRP-conjugated Polyclonal Goat Anti-Rabbit IgG(H+L) Secondary Antibody | ||
| AMre80004 | GAPDH (12R9) Rabbit Monoclonal Antibody |
Related Products
References
- Christofferson DE, Yuan J. Necroptosis as an alternative form of programmed cell death. Curr Opin Cell Biol. 2010 Apr;22(2):263-8. [PMID: 20045303].
- Degterev A, Hitomi J, Germscheid M, Ch'en IL, Korkina O, Teng X, Abbott D, Cuny GD, Yuan C, Wagner G, Hedrick SM, Gerber SA, Lugovskoy A, Yuan J. Identification of RIP1 kinase as a specific cellular target of necrostatins. Nat Chem Biol. 2008 May;4(5):313-21. [PMID: 18408713].
- Dondelinger Y, Declercq W, Montessuit S, Roelandt R, Goncalves A, Bruggeman I, Hulpiau P, Weber K, Sehon CA, Marquis RW, Bertin J, Gough PJ, Savvides S, Martinou JC, Bertrand MJ, Vandenabeele P. MLKL compromises plasma membrane integrity by binding to phosphatidylinositol phosphates. Cell Rep. 2014 May 22;7(4):971-81. [PMID: 24813885].
 | Flora Flora is a technical support expert at EnkiLife, familiar with immunology and neuroscience, dedicated to providing customers with high-quality product combinations and technical support to help achieve research in neurodegenerative diseases and other neuroscience areas. |