PROTAC E3 Ligase Profiling Antibody
PROTAC (proteolysis-targeting chimera) is a technique that uses a class of small molecules to target specific proteins for degradation. The small molecules are heterobifunctional, consisting of two protein binding parts joined by a linker. One part of the molecule binds a protein of interest (POI), while the other part binds to an E3 ubiquitin ligase, bringing the E3 ligase close to the POI for ubiquitination coupled protein degradation. The first successful PROTAC study used the chimeric PROTAC-1 molecule to recruit the β-TrCP E3 ligase to dictate the ubiquitination and degradation of MetAP2. There are over 600 E3 ligases, and only a few have been successfully developed by PROTAC to degrade target proteins. Among them, CRBN- and VHL-based PROTAC have been extensively explored and successfully applied for multiple disease treatments. The E3 ligased MDM2, c-IAP, and XIAP are fruitful in using PROTAC strategy for cancer treatment. PROTAC design using the KEAP1 E3 ligase has been developed for degradation of BRD3, BRD4, and Tau. KEAP1 is another promising member of the PROTAC team.
The action mechanism of PROTACs, mainly refers to recruitment, ubiquitylation, degradation, and recycling.
Relevant Antibodies
| Catalog# | Product Name | Application | Reactivity |
|---|---|---|---|
| APRab05622 | VHL (phospho Ser68) Rabbit Polyclonal Antibody | IHC-P,IF-P,IF-F,ICC/IF,ELISA | Human,Mouse,Rat |
| AMRe21357 | Keap1 Rabbit Monoclonal antibody | WB,IHC,IF,IP,ELISA | Human,Mouse,Rat |
| AMRe21162 | MDM2 Rabbit Monoclonal antibody | WB,IHC,IF,IP,ELISA | Human,Mouse,Rat |
| AMM81896 | BTRC Mouse Monoclonal Antibody | WB,IHC,FC,ELISA | Human |
| AMRe21157 | XIAP Rabbit Monoclonal antibody | WB,IF,IP,ELISA | Human,Mouse,Rat |
| APS0635 | HRP-conjugated Polyclonal Goat Anti-Rabbit IgG(H+L) Secondary Antibody | ELISA,WB,Dotblot | Mouse |
| AMRe80004 | GAPDH (12R9) Rabbit Monoclonal Antibody | WB,ELISA | Human,Mouse,Rat,Rabbit,Dog,Monkey |
Related Products
- Antibody Labeling Kit
- Western Blot Kits
- Super-sensitive ECL chemiluminescent reagent
- IHC Kit
- TSA mIHC Kits
References
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 | Voisey Voisey is a technical support specialist at EnkiLife, proficient in immunology and cell biology. She is committed to providing customers with professional and efficient technical support. Additionally, she is involved in research on customers' fields of study and designs highly cost-effective solutions for them. |