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By integrating single-cell transcriptomics and spatial transcriptomics technologies, it constructs a spatially resolved cell atlas of gastric cancer, aiming to systematically reveal the spatial organization characteristics of the tumor microenvironment, the core role of tertiary lymphoid structures, and the spatial distribution patterns of immune checkpoint molecules. This provides a new perspective for the exploration of biomarkers for gastric cancer immunotherapy and a scientific basis for the formulation of clinical individualized immunotherapy strategies.