Product Description
The Mouse CD4+ T Cell Isolation Kit (positive selection) is designed to isolate CD4+ T cells from mouse splenocytes or single-cell suspensions of other tissues. The principle utilizes CD4 Capture Antibody to label CD4+ cells, followed by capture with Releasable EnkiBeads™. The EnkiBeads™ Release Buffer is then used to dissociate the magnetic beads from the cell surface, yielding bead-free mouse CD4+ cells. The isolated CD4+ cells can be used for downstream molecular and cellular biology applications.
Storage Conditions and Shelf Life
Storage Conditions: Store at 2–8°C. DO NOT FREEZE.
Shelf Life: 2 years
Applications
This kit is suitable for isolating CD4+ T cells from mouse spleen and lymph nodes.
Precautions
Avoid freezing magnetic beads and antibody mix during storage and use.
Use low-retention pipette tips and centrifuge tubes to minimize loss of beads and antibodies due to adsorption.
This product should be used with a magnetic separator.
For research use only.
