Product Description
The Mouse CD4+ T Cell Isolation Kit is designed to isolate CD4+ T cells from mouse splenocytes or single-cell suspensions of other tissues via negative selection. The principle involves labeling non-target cells (non- CD4+ T cells) with biotin-conjugated monoclonal antibodies, followed by depletion using streptavidin-conjugated magnetic beads. This process enriches mouse CD4+ T cells. A magnetic separator is required for the procedure.
Storage Conditions and Shelf Life
Storage Conditions: Store at 2–8°C. DO NOT FREEZE.
Shelf Life: 2 years
Applications
This kit is suitable for isolating CD4+ T cells from mouse spleen and lymph nodes.
Precautions
Avoid freezing magnetic beads and antibody mix during storage and use.
Use low-retention pipette tips and centrifuge tubes to minimize loss of beads and antibodies due to adsorption.
This product should be used with a magnetic separator.
For research use only.
