Product Description
EnkiLife His-tag protein purification beads possess superparamagnetism, it is a new functional material designed for efficient and rapid purification of His-tag protein. It can extract the target protein with high purity from the biological samples in one step using magnetic separation method and it greatly simplifies the purification process and improves the efficiency of purification. This method is suitable for scientific research and industrial areas to facilitate the purification of his-tag protein.
Compared with the traditional tomographic method which use metal chelate agarose prepacked column, EnkiLife His-tag Protein Purification beads do not need to carry out high-speed centrifugation and filter filtration on the crude protein samples for a long time, or control the flow rate, and do not need the expensive chromatography equipment. The specific binding of the sample to the magnetic beads, following washing and target protein elution become very simple, fast and easy to operate. For skilled operators, highly purified protein can be obtained within 1 hour, and parallel processing of high throughput and large amount samples can be easily achieved to save researchers time and cost.
The His-tag product line includes two metal ion chelate beads consist of Nickel and Cobalt, they have different properties at combining ability of target proteins and nonspecific adsorption. In addition, users can choose different metal ion chelate beads from different purpose
Storage Conditions and Shelf Life
Storage Conditions: Store at 2–8°C. DO NOT FREEZE.
Shelf Life: 2 years
Precautions
Be sure to read this user's manual carefully before using this product for the first time;
The freezing, drying and high-speed centrifugating and other operations should be avoided during the usage and storage of the beads ;
Before using this product, be sure to fully oscillate the beads to maintain a uniform suspension;
Please use pipette tip and centrifuge tube with good quality to avoid the stick on the wall or ENKILIFE beads loss caused by tube leakage during the mixing process;
In the process of mixing the beads and the solution, if the solution is viscous, the magnetic beads cannot be resuspended by fliping the centrifuge tube, using short-term vortex mixing to make the ENKILIFE beads fully resuspended;
Users can retain the removed supernatant to analyze the purification process and optimize the protein purification process;
This product can be reused. When the purification performance is reduced, it is recommended to regenerate beads.
When the used magnetic beads are reused, it is recommended to purify the same kind of proteins, when purifying different kinds of proteins, it is recommended to use new magnetic beads;
This product must be used with magnetic separation equipment.
This product is for research use only.
